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Our phenotyping department is equipped with a dedicated viral transduction facility and highly trained staff for delivery of viral vectors. A large panel of delivery route are validated for gene transfer in adult mouse. Other routes, delivery in young mice or in rats can be developed upon request. Biodistribution and efficiency analyses and downstream phenotyping tests can be performed on transfected animals

Vectors frequently used:

  • Adenovirus
  • AAV
  • Lentivirus
  • LentiFlash particles (for non-integrative RNA delivery)
  • Vaccinia

Delivery modes currently available:

  • Intradermic; Subcutaneous
  • Intravenous (retro orbital or jugular vein injection)
  • Intraperitoneal
  • Stereotactic – intraventricular and cerebellar
  • Intramuscular
  • Intrajugular slow injection – minipump
  • Intranasal aerosol
  • Intratumoral
  • Intratracheal

Sample results

Gene-Expression_viral-transduction-Photo1-V2.png

Intramuscular injection of cre vector in mT/mG reporter mouse (Muzumdar, Mandar Deepak, Bosiljka Tasic, Kazunari Miyamichi, Ling Li, and Liqun Luo 2007- A Global Double-Fluorescent Cre Reporter Mouse. Genesis (New York, N.Y.: 2000) 45(9): 593–605.)

Legend: green: recombined cells; red: non recombined cells