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A large panel of delivery route are validated for gene transfer in adult mouse. Other routes, delivery in young mice or in rats can be developed upon request. Biodistribution and efficiency analyses and downstream phenotyping tests can be performed on transfected animals
Intramuscular injection of cre vector in mT/mG reporter mouse (Muzumdar, Mandar Deepak, Bosiljka Tasic, Kazunari Miyamichi, Ling Li, and Liqun Luo 2007- A Global Double-Fluorescent Cre Reporter Mouse. Genesis (New York, N.Y.: 2000) 45(9): 593–605.). Legend: green: recombined cells; red: non recombined cells
Stereotactic delivery are widely used for brain manipulation regarding neurodegenerative disorders. With the development of gene therapy, the injection of viral vectors into specific brain structures is admitted to be a valuable technique in the study of treatments for neurodegenerative brain diseases (Parkinson's, Alzheimer's...).
Legend: cerebellum mouse slice, stained with an HA antibody to detect « confidential » transgene expression after intra cerebellar AAV delivery using stereotactic technics. Francoise Piguet ©IMC
With automated stereotactic instruments and a microbiological safety station, we have been performing intracerebral injections of AAV followed by behavioral studies in the mouse models. Finally, we recently succesfully conducted a preliminary toxicity study on rats to confirm those obtained in mice.
Legend : automated intracerebral injection system for mouse and rat models.