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Our phenotyping department is equipped with a dedicated viral transduction facility and highly trained staff for delivery of viral vectors. A large panel of delivery route are validated for gene transfer in adult mouse. Other routes, delivery in young mice or in rats can be developed upon request. Biodistribution and efficiency analyses and downstream phenotyping tests can be performed on transfected animals

Vectors frequently used:

  • Adenovirus
  • AAV
  • Lentivirus
  • LentiFlash particles (for non-integrative RNA delivery)
  • Vaccinia

Delivery modes currently available:

  • Intradermic; Subcutaneous
  • Intravenous (retro orbital or jugular vein injection)
  • Intraperitoneal
  • Stereotaxic admininistration – intraventricular and cerebellar
  • Intramuscular
  • Intrajugular slow injection – minipump
  • Intranasal aerosol
  • Intratumoral
  • Intratracheal

Intramuscular injection : sample results

Gene-Expression_viral-transduction-Photo1-V2.png

Intramuscular injection of cre vector in mT/mG reporter mouse (Muzumdar, Mandar Deepak, Bosiljka Tasic, Kazunari Miyamichi, Ling Li, and Liqun Luo 2007- A Global Double-Fluorescent Cre Reporter Mouse. Genesis (New York, N.Y.: 2000) 45(9): 593–605.). Legend: green: recombined cells; red: non recombined cells

Stereotaxic admininistration  for mouse and rat

Purpose:

Stereotaxic delivery are widely used for brain manipulation regarding neurodegenerative disorders. With the development of gene therapy, the injection of viral vectors into specific brain structures is admitted to be a valuable technique in the study of treatments for neurodegenerative brain diseases (Parkinson's, Alzheimer's...).

Sample results:

Gene Expression_Stereotaxic_Marquage-Cervelet.png

Legend: cerebellum mouse slice, stained with an HA antibody to detect « confidential » transgene expression after intra cerebellar AAV delivery using stereotactic technics. Francoise Piguet ©IMC

Equipment:

With automated stereotaxic instruments and a microbiological safety station, we has been performing intracerebral injections of AAV followed by behavioral studies for more than a year in the mouse models. Finally, we recently succesfully conducted a preliminary toxicity study on rats to confirm those obtained in mice.

  • Automated stereotaxic instruments (World Precision Instruments, United States)
  • Microbiological safety station

Tools-Automated Stereotax-2-1100px.png

Legend : automated intracerebral injection system for mouse and rat models.