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This technology relies on transgene integration in the targeted Rosa26 locus to overcome classical random transgenesis drawbacks.


This is achieved through homologous recombination in ES cells of a targeting vector containing the transgene (conditional in most of the cases) and Rosa26 homology arms. Alternative docking sites are Hprt or Col1a1.



  • Transgene insertion in a specific locus
  • Integration of a single-copy transgene; this minimizes the risk of silencing by methylation as the Rosa26 has been used extensively for transgene expression.
  • Transgene consistent levels of ubiquitous expression driven either by the endogenous ROSA26 promoter in the embryo or by a synthetic CMV enhancer-chicken b-actin promoter (pCAG) in later stages; all founder lines will exhibit similar expression level


  • This method cannot be used to generate knock-out or knock-in models

PHENOMIN expertise

  • Personalized genome engineering service to fit our customer needs
  • Implication in international consortia (near 20 different consortia) to benefit from the last technics and scientific expertise
  • Continuous R&D activity to improve our technics and success rate and propose our customers the best approach for their projects