Latest News

ANNOUNCE Meet us and get advices at 8th Charles River Short Course
March 20, 2017

Meet us and get advices at 8th Charles River Short Course

PHENOMIN's team will attend the course and will share its expertise for increasing your awareness regarding the advantage and limits of the CRISPR-CAS9 genome editing technics in vivo and for helping you in improving the reproducibility of your scientific data with in vivo studies.

More information at http://eushortcourse.criver.com/sc-agenda.html


WEBINAR CRISPR/Cas9 in Rodents
March 9, 2017

PHENOMIN-ICS will present a webinar "CRISPR/Cas9: The Good, the Bad and the Ugly", March 30, 2017, 3:00 PM CEST/9:00 AM EST

In this session, we will present

  • our results for generating complex mouse or rat models.
  • the actual limitations of this system for the modification of “big” genomic sequences 
  • the crucial request of careful validation of the lines generated by CRISPR/Cas9 

Presenters

  • Guillaume Pavlovic, PhD, Head of the Genetic Engineering and Model Validation Department, PHENOMIN-ICS
  • Jean Cozzi, PhD, Embryology Laboratory Manager, Charles River

Register at: http://www2.criver.com/l/60962/2017-03-08/ch9pxs


JOINT COURSE 2nd Programmable nucleases (CRISPR/Cas9) Hands-on Transgenesis Course
Feb. 14, 2017

2nd Programmable nucleases (CRISPR/Cas9) Hands-on Transgenesis Course

DEAD line: February 15th !

The course is jointly provided by the Czech Centre for Phenogenomics and PHENOMIN/ICS Strasbourg, both members of INFRAFRONTIER, is designed to give practical, hands-on training in current technologies and methods employing CRISPR/Cas9-mediated genome manipulations in the generation of mice mutants. Participants will gain theoretical and practical knowledge from our expert instructors, have ample opportunity for discussion and can bring their own targeting projects to work on during the bioinformatics session. The course has been designed for participants with an intermediate knowledge of molecular biology.

Download


CALL INFRAFRONTIER open call "Disease model development and systemic phenotyping"
Jan. 17, 2017

INFRAFRONTIER open call "Disease model development and systemic phenotyping"

INFRAFRONTIER, the European Research Infrastructure for phenotyping and archiving of model mammalian genomes is opening a call to facilitate access for the wider biomedical research community to the unique infrastructure and scientific expertise of the INFRAFRONTIER mouse clinics. INFRAFRONTIER offers the testing of mouse mutant lines through a broad based primary phenotyping pipeline in all the major adult organ systems and most areas of major human disease. Access will be granted on the basis of scientific excellence and supports the development and in depth characterisation of new mouse models for investigating gene function and human pathophysiology. INFRAFRONTIER will provide open access to all newly developed disease models and phenotyping data.

 

More information and call application form:

https://www.infrafrontier.eu/resources-and-services/infrafrontier-open-calls/model-development-and-systemic-phenotyping-call

Proposal submission to proposals@infrafrontier.eu by 15 February 2017.

Proposal evaluation from 16 February to 31 March 2017.


Upcoming events

MEETING 4th Conference of Translational Medicine on the Pathogenesis and Therapy of Immune-Mediated Diseases
March 27 - 29, 2017
Palermo, Italy

The 4th Conference of Translational Medicine on the Pathogenesis and Therapy of Immune-Mediated Diseases is an opportunity for researchers and medical doctors interested in the pathogenesis and novel therapeutic approaches of human immunologic disease to get together. The meeting will focus on innate immunity and inflammation in three models of human diseases: Autoinflammatory/Autoimmune Disorders/PID, Cancer and Viral Infection. As in 3 the past conferences, we are going to have a translational approach to all topics. The meeting will host 5 lectures from 5 pioneers in immunology and will have a prestigious faculty of invited speakers composed by the top scientist in the related research field that already confirmed their participation. 

Meet us and get advices

Guillaume Pavlovic from PHENOMIN-ICS will presente "CRISPR/Cas9 for rodents models of human disease: the good, the bad and the ugly" on Monday 27th march  at 16:40. 

More information at http://www.translationalimmunology.it/


TRAINING COURSE Mycoplasmes detection in cell culture
March 29, 2017
Illkirch, France

Objectives

  • Realize the importance of tracability and quality control in cell culture
  • Be aware of the problem of mycoplasma contamination
  • Realize the diversity of mycoplasma detection methods and be able to choose techniques adapted to each case
  • Know how to prevent mycoplasma contaminations

Audience

Engineers and technicians currently cultivating cell lines or planning to cultivate cell lines.

Prerequisites

None, except understand French (course will be given in French)

Program

Interactive courses (5h)

  • Introduction on cell line culture
  • How to establish cell lines and key steps in the culture
  • Importance of tracability and quality control
  • Mycoplasma: definitions and classifications- origin of contamination- effects of contamination- detection methods- treatment and limits- preventions and actions needed

Workshop 2 h

Starting from concrete cases, trainees will be asked to analyze the situation in order to suggest an action plan


Registration and more informations: Download the program and pre regristration form

PHENOMIN's contact: wattenho@igbmc.fr

More information at https://cnrsformation.cnrs.fr/stage-17280-Detection-des-mycoplasmes-dans-les-cultures-de-lignees-cellulaires.html?mc=mycoplasmes


SPECIAL EVENT Webinar CRISPR/Cas9 in Rodent Animals
March 30, 2017
On line

CRISPR/Cas9: The Good, the Bad and the Ugly
March 30, 2017
3:00 PM CEST/9:00 AM EST

In the past years, CRISPR/Cas9 has shown to be a real revolution for generation of genetically engineered animals and a real hope for genetic therapy in humans. This powerful genome editing technology is working in a very large panel of species from plants to mammals. In rodents, numerous knock-out or point mutations models were easily generated with this approach. Among other advantages, it is fast (to obtain founder animals), quite easy (in comparison to ES route) and cost-effective. It also allows accessing a large panel of background and thus promise to be a powerful tool for polygenic studies.

In this session, we will present our results for generating complex mouse or rat models. We developed the CRISPR Mediated REarrangement (CRISMERE) strategy, which takes advantage of the CRISPR/Cas9 system, to generate easily most of the desired structural variant & CNV rearrangements. We were indeed able to achieve deletions, duplications, and inversions of genomic regions as large as 24.4 Mb in rat and mouse (the good).

We also used CRISPR/Cas9 to develop knock-in or conditional lines. We will show the actual limitations of this system for the modification of “big” genomic sequences in mouse and rat (the bad) and discuss possible solutions and explanations.

Finally, we will also show that very careful validation of the lines generated by CRISPR/Cas9 is requested (the ugly) and how some unexpected event can be used to generate targeted overexpressing models.

Presenters

  • Guillaume Pavlovic, PhD, Head of the Genetic Engineering and Model Validation Department, PHENOMIN-ICS
  • Jean Cozzi, PhD, Embryology Laboratory Manager, Charles River

To register

http://www2.criver.com/l/60962/2017-03-08/ch9pxs

 

More information at http://www2.criver.com/l/60962/2017-03-08/ch9pxs


TRAINING COURSE 2nd Programmable Nucleases Course
April 3 - 7, 2017
Vestec, Czech Republic

This course, jointly provided by the Czech Centre for Phenogenomics and PHENOMIN/ICS Strasbourg, both members of INFRAFRONTIER, is designed to give practical, hands-on training in current technologies and methods employing CRISPR/Cas9-mediated genome manipulations in the generation of mice mutants. Participants will gain theoretical and practical knowledge from our expert instructors, have ample opportunity for discussion and can bring their own targeting projects to work on during the bioinformatics session. The course has been designed for participants with an intermediate knowledge of molecular biology.

More information at https://www.phenogenomics.cz/2016/12/transgenesis-course-in-april/


CONGRESS French Society of Cardiology- PC 2017
April 6 - 7, 2017
Nantes, France

Rendez-vous à Nantes pour le Printemps de la Cardiologie 2017

Cette année le printemps sera Nantais !

Nantes, riche de son histoire (bretonne?), de ses produits du terroir, de sa gastronomie et de la chaleur de son accueil, saura vous combler avec ses spécialités, ses vins et ses « rigolettes ».

Venir à Nantes c’est déjà un avant-gout du grand large… L'enthousiasme, l'audace, la générosité et le jeu collectif, Nantes en fait sa marque de fabrique pour le fameux jeu à la nantaise.

Il n’en faut pas moins pour construire un programme scientifique attractif qui saura combler aussi bien les scientifiques que les cliniciens dans une volonté affirmée de translationalité propice aux échanges. Pour mieux préparer la recherche médicale de demain, les nouveautés technologiques, la médecine connectée et le traitement de l’information seront mis à l’honneur.

Les jeunes sont le cœur du Printemps. Nantes les accueillera dignement que ce soit pendant le congrès avec un parcours qui leur sera entièrement dédié ou lors de la soirée du GRRC qui s’annonce déjà inoubliable.

Participer au Printemps de la Cardiologie, le plus grand congrès de recherche cardiovasculaire francophone, c’est s’ouvrir à la nouveauté, rencontrer une communauté dynamique et créer de nouvelles opportunités de projets.

Au nom du Groupe de Réflexion pour la Recherche Cardiovasculaire et de la Société Française de Cardiologie et du comité scientifique et d’organisation, nous vous souhaitons un Printemps de la Cardiologie riche de rencontres et d’innovations.

More information at http://www.printemps.sfcardio.fr/


WORKSHOP CELPHEDIA Workshop Meeting 2017
May 4, 2017
Paris, France

CELPHEDIA is a network including fifteen centers spread throughout the French territory to be closer to the users. This distributed operational RI (Research Infrastructure) gathers a remarkable palette of expertise, skills and knowledge unique in Europe, in the domains of creation, functional exploration, archiving and distribution of organisms, used as models for fundamental research and preclinical approaches: rodents with the mouse as a leader, non-human primates and non mammalians including aquatic vertebrates. 

CELPHEDIA Network gathering work packages composed of experts in their respective field, proposes to every WP member to meet during CELPHEDIA Workshop Meeting 2017

The aim of the workshop is to continue the development of the current collaborative cross-center projects, but also to optimise and harmonise protocols and processes in the areas specific to each WP.

The Operational Committee is divided in workpackages following 4 main topics:

  • Management & Communication
  • Genome modification
  • Zootechnics, ethics & animal welfare
  • Phenotyping & multi-species approaches

The working groups correspond to the objectives of CELPHEDIA nodes and gather all the scientific experts of the network in a given area.

Due to its unique combination of expertise and skills, CELPHEDIA enables to develop transverse inter-species approaches, at the level of both the technology and the study of human diseases.

On one hand, these WPs materialize this combination of skills through exchanges between the centers. On the other hand, they enable the harmonization of protocoles and processes, either scientific or administrative, to provide users with services specific to the network through CELPHEDIA gateway.

CELPHEDIA Workshop Meeting 2017 will take place  at CNRS headquarters, 3 Rue Michel-Ange in Paris.

More information at http://www.celphedia.eu/


SPECIAL EVENT 2d European Advanced School for Mouse Phenogenomics
June 12 - 16, 2017
Goersdorf, France

Would you like to learn how to choose, create and analyse your best mouse model?

Do you have a sufficient background in mouse functional genomics to lead as best your research?

Then, come to the heart of Europe to be involved - together with other students and researchers from all over the world - in opened discussion, in constructive analysis of your own research and in building conceptual scientific advances.This unique international event will provide the attendees with an innovative program focused on the best practices in the use of the mouse model for biomedical and basic research

 

24 experts from European landscape will be present to:

  • Exchange and discuss your major on-going works, scientific and methodological issues.
  • Share knowledge and expertise, transfer new concepts and innovative methods

You will gain crucial skills required to use as best mutant mice (best experimental design, scientific and ethic consideration for you best experimental design, awareness for improving robustness of your scientific data,..)

The target audience consists of engineers and/or researchers who directly or indirectly use mouse as animal model for scientific research.

We encourage both industrial and academic researchers, postdocs and PhDs from all Europe to attend this school.

 

CNRS researchers: FREE of charge

PhD applicants: 360€ (FREE of charge for the students from the University of Strasbourg and University of Aix-Marseille)

Applicants from governmental institutions: 600€

Applicants from private institutions or companies: 1680€

 

Deadline for application : April 28th 2017 (Early bird dead line : March 31st)

More information on program and registrationhttps://advanced-school.phenomin.eu/ 

Contact advanced-school@phenomin.eu 

More information at https://advanced-school.phenomin.eu/


TRAINING COURSE Small animal imaging: evolution, potential, limits
November 6 - 10, 2017
Orleans, France

Objectives:

  • Understand potential and limits of different imaging modalities for small animal: biophotonic imaging (bioluminescence, fluorescence 2D and 3D), X rays (X scanner), echography coupled to photoaccoustic
  • Be able to select the most suitable approach to address a biological question (phenotyping, functional exploration, biopharmaceutical research...)

The course is conducted in French

Acces to detailed program and registration procedure

CNRS formation entreprises

PHENOMIN's contact:
stephanie.lerondel@cnrs-orleans.fr 

More information at http://cnrsformation.cnrs.fr/stage-17142-Imagerie-du-petit-animal--evolution%2C-potentiel%2C-limites.html?mc=imagerie


MEETING 9 ieme colloque des belles souris
November 9, 2017
Toulouse, France

Le but du Club des Belles Souris est de susciter des échanges scientifiques autour des technologies et techniques en génétique moléculaire de la souris. 

Le programme scientifique du 8 ième Colloque est ouvert !

Vous pouvez vous inscrire dès maintenant en utilisant le site web

More information at http://stemcells.free.fr/?page_id=1175


TRAINING COURSE In vivo CRISPR-Cas9 genome editing
November 9 - 10, 2017
Illkirch, France

Objectives

PHENOMIN's training aims to provide a general framework to get scientists started using CRISPR/Cas9 for in vivo gene editing in rodents.

The main topics are:

  • Learn more about gene editing and how it works
  • Hear about current advances on many technical aspects
  • Optimize the RNA guide design to the genotyping analysis (bioinformatics workshop).
  • Highlight crucial issue in your own scientific project.

Acces to Detailed program and registration procedure

CNRS formation entreprise

PHENOMIN's contact:
bedu@igbmc.fr

More information at http://cnrsformation.cnrs.fr/stage-17276-In-vivo-CRISPR-Cas9-genome-editing.html?mc=CRISPR-Cas9