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12nd Call - Fondation maladie rares

Development of experimental models for rare diseases

The project had to be based on scientifically validated preliminary data and the choice of the experimental model must be clearly justified. The call was dedicated only to generating new experimental models for rare diseases i) animal models, ii) in vitro models based on induced pluripotent stem cell (iPSC) (including conditions for archiving lines). Any other request (breeding, phenotyping, advanced imaging etc.) was not eligible.

Animal models

To develop the new experimental animal models of rare diseases, successful applicants will have a facilitated access to experimented platforms for which most of them are being part of CELPHEDIA, the French infrastructure that promotes innovative services and tool on model organisms:

  • Non-mammalian models
  • Mammalian models: Rabbit, Rat, Mouse

The precise type of model development (knock-out, knock-in, humanized model, transgenic…), will be specific to each organism, but will rely on the latest improvements and most appropriate techniques of genome editing (ZFNs, TALEN, CRISPR/CAS9, etc.) or more classical transgenic approaches (such as DNA microinjection or lentiviral infection). 

Human induced pluripotent stem cell (iPSC) lines

For the development of in vitro models based on induced pluripotent stem cell (iPSC) lines, the best suited approach will be designed for each project. Please pay attention that only projects for which agreements of lineage derivation from iPS of patients have already been obtained will be considered.  Human induced pluripotent stem cell (iPSC) lines will be developed with the support of the Platform iPSC Nantes.  

 

More information regarding this pasted call at aap-bio@fondation-maladiesrares.com  

11st Call - INFRAFRONTIER

INFRAFRONTIER2020 Project - Trans-national Access call - (January 2019)

Precision mammalian model development / rat models - January 2019

This Trans-national Access call of the INFRAFRONTIER2020 project supports a total of 3 precision rat model development projects. A complementary call provides support for 12 customised mouse model development projects.

The EC Horizon 2020 funded INFRAFRONTIER2020 project (2017 – 2020) supports eligible customers by providing a total of 5 access units in this call.The access unit offered covers the derivation of germ-free (axenic) mice from a breeding nucleus or from frozen materials provided by the applicants. Mice will be kept under germ-free conditions for 6-8 weeks.

More information about this call

10th call - INFRAFRONTIER

INFRAFRONTIER2020 Project - Trans-national Access call - (December 2018)

Derivation of germ-free mice (axenic service)

This call offers a service to derive germ-free mice for European researchers who study the role of the microbiome in metabolism, physiology, and immune function.The `axenic´ service is free of charge. Access to the axenic service will be granted on the basis of scientific excellence and supports pilot projects for the derivation of-germ-free mice. Further breeding and characterisation of axenic mice or the development of gnotobiotic models can be offered on a fee-for-service or on a collaborative basis.

The EC Horizon 2020 funded INFRAFRONTIER2020 project (2017 – 2020) supports eligible customers by providing a total of 5 access units in this call.The access unit offered covers the derivation of germ-free (axenic) mice from a breeding nucleus or from frozen materials provided by the applicants. Mice will be kept under germ-free conditions for 6-8 weeks.

More information about this call

9th call - INFRAFRONTIER

INFRAFRONTIER2020 project - Trans-national Access call - (October 2018)

Specialised Phenotyping - metabolic and behavioral phenotyping pipelines

Main objective of this INFRAFRONTIER2020 Trans-national Access call is to facilitate access for the wider biomedical research community to the unique infrastructure and scientific expertise of the participating INFRAFRONTIER mouse clinics, where mouse mutant lines can be tested through specialised metabolic- and behavioural phenotyping pipelines. Disturbed energy balance regulation results in obesity and in numerous related metabolic disorders such as type 2 diabetes mellitus. We offer specialised phenotyping pipelines comprising state-of-the-art test assays monitoring disease related parameters. In-depth behavioural phenotyping supports the elucidation of the molecular and genetic basis of behavioural impairments that are relevant for human neuropsychiatric disorders such as anxiety disorders, post-traumatic stress disorder, depression, schizophrenia, autism, attention-deficit hyperactivity disorder, Parkinson’s and Alzheimer’s Disease.

Access will be granted on the basis of scientific excellence and supports the development and in depth characterisation of mouse models for investigating gene function and human pathophysiology.  INFRAFRONTIER will provide open access to all characterised disease models and phenotyping data.

More information about this call

8th call - INFRAFRONTIER

INFRAFRONTIER2020 project - Trans-national Access call- (April 2018)

Precision mammalian model development / rat models

Rat models for studies in neurobiology, cardiobiology, and immunology

Main objective of this INFRAFRONTIER open call is to facilitate access for the wider biomedical research community to the unique infrastructure and scientific expertise of the participating INFRAFRONTIER partners, to deliver novel rat mutant models that will advance knowledge of human disease and will be of widespread use in biomedical science. Many cognitive and physiological characteristics make the rat an ideal human disease model and choice for laboratory studies in neurobiology, cardiobiology, and immunology. Recent advances in genome-editing technology will be used to develop new rat models of human disease.

INFRAFRONTIER will provide open access to all newly developed disease models through the European Mouse Mutant Archive (EMMA). Access to this free-of-charge-service will be granted on the basis of the applicant’s research plans and the potential impact of the proposed novel rat model on the wider biomedical research community.

More information about this call

7th call - Fondation maladies rares

The French Foundation for rare diseases "Fondation maladies rares" and the French National Infrastructure PHENOMIN are pleased to launch their 3rd joint call for the creation and exploration of mouse models for rare diseases.
The call for projects 'Mouse models and rare diseases' aims to give a significant boost to the development of mouse models, in order to:

  • gain a better understanding of the pathophysiological mechanisms involved in rare diseases whose defective genes have been identified;
  • test and validate therapeutic proofs of concept, at the pre-clinical in vivo level.

Indeed, producing these models meets a key objective in the development of a therapeutic strategy. After their initial in vitro testing, therapeutic proofs of concept must be tested in a living model that recapitulates as closely as possible both the phenotype and biological defects associated to the human disease. Such a model should provide appropriate data regarding the safety and the efficiency of the drug, thus evaluating its benefit/risk ratio, prior to conducting early phases of a therapeutic trial. PHENOMIN and the French Foundation for rare diseases combine their efforts in order to achieve these objectives through the joint call for proposals for the generation and characterization of mouse models, dedicated to rare diseases. This action is part of the objectives of PHENOMIN to develop mouse model resources that will be made available to the scientific community.
 
Upload the call for proposal 2016-MousemodelsRareDiseases-final.pdf

Submission deadline for proposals: February 9, 2017, 5:00 pm
 
On line submission: HERE.

6th Call - IMPC

We are glad to announce the new PHENOMIN call dedicated to generation and characterization of CreERT2 cell specific promoter driven deleter mouse lines.

Since 2011, PHENOMIN has generated more than 150 models with conditional potential. In the frame of the International Mouse Phenotyping Consortium (IMPC) more than 5,000 genes are already committed to the IMPC pipeline. Most of these models can be driven in conditional alleles.  

We aim to maximize the utility of this resource by offering the scientific community with the possibility to  nominate the making of new Cre or CreERT2 lines.

In the last decade, we have generated and validated more than 30 cell specific inducible CreERT2 deleter lines (http://mousecre.phenomin.fr/).  We have written guidelines for using cre/lox system in mice (European FP7 CREATE program, http://www.creline.org/other_cre_db_resources). We developed a highly efficient universal cre deleter line (http://www.ncbi.nlm.nih.gov/pubmed/22121025). As a partner in EUCOMTOOLS effort, we are establishing new 250 CreERT2 knock-in models (http://www.mousephenotype.org/about-ikmc/eucommtools) in a pure C57BL/6N background.

The call targets the French scientific community. It is a real opportunity to nominate new CreERT2 drivers with useful expression patterns. Projects will be selected on scientific bases and non-availability of similar Cre/CreERT2 lines available in other repositories. Proposals will be reviewed by a scientific committee.

Application deadline is 15th of October 2015.

 

Publication of results :

The National Infrastructure PHENOMIN is pleased to announce the results of its 6th Call: Generation and Characterization of CreERT2 cell specific promoter driven deleter mouse lines. The Scientific Committee has selected 13 projects amongst 34 submitted projects from 20 PI. Consequently, 9 additional CreERT2 mouse lines will be generated by PHENOMIN team and will be made available as soon as possible for the scientific community.
The 9 selected models are based on (in alphabetical order):

  • Clec4f (MGI:1859834) to target CreERT2 in Kuppfer Cells (liver)
  • Fshr (MGI:95583) to target CreERT2 in ovarian granulosa cells
  • Mcpt8 (MGI:1261780) to target CreERT2 in basophils
  • Mtnr1b (MGI:2181726) to target CreERT2 in melatonin responsive cells
  • Nr2f2 (MGI:1352452) to target CreERT2 in the fate regulation
  • Pitx2 (MGI:109340) to target CreERT2 in Subthalamic nucleus
  • Prg2 (MGI:103294) to target CreERT2 in eosinophils
  • Scgb3a2 (MGI:2153470) to target CreERT2 in Clara cells (lung bronchi)
  • Tlr5 (MGI:1858171) to target CreERT2 in cells expressing TLR5 (flagellin receptor)

4 CreERT2 mouse models have been already generated by the European EUCOMMTOOLS consortium and will be further characterized (in alphabetical order):

  • Cd4 (MGI:88335) to target CreERT2 in CD4 T-cells
  • Itgax (MGI:96609) to target CreERT2 in dentritic cells
  • Jchain (MGI:96493) to target CreERT2 in plasma cells
  • Slc12a3 (MGI:108114) to target CreERT2 in distal nephron

To get more information, contact mutagenesis@igbmc.fr

Thanks again to all the participants !

 

5th Call - IMPC

We are glad to announce the 5th PHENOMIN call for proposals for conditional or constitutive knock-out mouse generation and standard phenotyping.

Since 2011, PHENOMIN has generated more than 150 models. In the frame of the International Mouse Phenotyping Consortium (IMPC) more than 5,000 genes are already committed to the IMPC pipeline.

Until the end of 2016, PHENOMIN will generate 100 additional knock-out lines. All these lines will be phenotyped through the standardized IMPC broad-based pipeline.

Up to now, all models have been generated from ES mutant cells with conditional potential generated by the International Knockout Mouse Consortium (IKMC).

In the frame of this 5th call, we have added constitutive knock-out models to be generated using CRISPR (clustered regularly interspaced short palindromic repeats) genome editing technology.

Conditions for eligibility:

  • For conditional and constitutive knock-out models: availability of at least 3 ES mutant cells with conditional potential generated by the IKMC consortium.
  • If no ES cells or less than 3 clones are available within the IKMC consortium, only the constitutive knock-out model will be generated using CRISPR genome editing technology.
  • To avoid any duplication within the IMPC, a gene already reserved by another center (i.e. already produced, or with production underway, or planned to be produced) will not be selected but the production state will be transferred to the requestor.

For all models that will be selected, please consider that:

  • As soon as the germ line transmission is confirmed, corresponding mouse lines will be available by the IMPC for your research as well as to any group which could be interested in.
  • The phenotyping data from comprehensive screen will be published on a public database and accessible to the whole scientific community.

Publication of results

The National Infrastructure PHENOMIN is pleased to announce the 5th Call for Proposals results for conditional or constitutive knock-out mouse generation and standard phenotyping. For the first time, the use of the CRISPR/Cas9 technology was included (KO models only).

Following the strong success of previous calls (more than 440 proposal submissions), the French academic scientific community has renewed its interest for phenogenomics.

The Scientific Committee has selected 39 projects amongst 46 submitted projects from 32 PIs.

Thanks to all scientists for your continuous interest.

4th Call - Fondation maladies rares

The French fondation for rare diseases "Fondation maladies rares" and the National Infrastructure PHENOMIN are pleased to announce the launch of the 2nd call for joint research projects: "Mouse models and rare diseases".

This call for projects 'Mouse models and rare diseases' aims to give a significant boost to the development of mouse models, in order to :

  • gain a better understanding of the pathophysiological mechanisms involved in rare diseases whose defective genes have been identified
  • test and validate therapeutic proofs of concept, at the pre-clinical in vivo level

Indeed, producing these models meets a key objective in the development of a therapeutic strategy. After their initial in vitro testing, therapeutic proofs of concept must be tested in a living model that recapitulates as closely as possible both the phenotype and biological defects associated to the human disease.
Such a model should provide appropriate data regarding the safety and the efficiency of the drug, thus evaluating its benefit/risk ratio, prior to conduct early phases of a therapeutic trial.

Icon Full text of the call for projects (PDF) (453.4 KB)

Icon Application form (Word) (227.5 KB)

You can also download these documents from the website of the Foundation for rare diseases (http://fondation-maladiesrares.org) by registering / logging in professional area, Call Projects section.

Research projects must be submitted via filled application form no later than January 13, 2015 5:00pm:

Each submitted form must be related to only one model.

We remain at your disposal for any further information and hope that you will respond to this call for many projects.
Wishing you every success in your projects.

 

Publication of results

The "Fondation maladies rares" in partnership with the National Infrastructure PHENOMIN, is supporting 13 research projects focusing on the development and the study of mouse models in the field of rare diseases.

The Joint Call "Mouse models and rare diseases" aims to give a significant impetus to the creation and the study of mouse models for rare diseases in order to elucidate underlying pathophysiological mechanisms and to develop new therapeutic approaches. This joint call is dedicated to the creation and the characterization of mouse models for rare diseases - constitutive Knock-Out (KO) / conditional Knock-Out (cKO), Knock-In (KI) and transgenic mice.

Icon List of projects (66.5 KB)

Platforms of PHENOMIN infrastructure which are partners of that Joint Call:

  • Institut Clinique de la Souris, Strasbourg
  • CIPHE, Marseille

3rd Call - IMPC

We are glad to announce the 3rd PHENOMIN expressions of Interest for generation and characterization of transgenic mouse lines bearing a single gene knock-out (with conditional potential).

Today, through its partnership with the International Mouse Phenotyping Consortium (IMPC) PHENOMIN has generated nearly 150 models and more than 5.000 genes are already committed to the pipeline of the IMPC.

 

Publication of results

The National Infrastructure PHENOMIN is pleased to announce the results of the 3rd Call for Proposals 2013: Establishing and characterizing mouse models - conditional Knock-Out (cKO).

The Scientific Committee has selected 45 projects amongst 98 submitted projects from 59 PI.
Consequently, 45 additional new mouse lines will be generated by PHENOMIN team, in order to be available as soon as possible for the scientific community.

IMPC Alleles: IKMC - EUCOMM/KOMP

IMPC_alleles

2nd Call - Fondation maladies rares

The French fondation for rare diseases "Fondation maladies rares" and the National Infrastructure PHENOMIN are pleased to announce the launch the call for joint research projects: "Murine models and rare diseases".

This call for proposals aims to establish and characterize mouse models: conditional Knock-Out (cKO), Knock-In (KI) and transgenic (TG) in the field of rare diseases. These knockout mice, together with the wealth of associated data, will serve as a valuable, freely available, resource for French researchers investigating gene function and human pathophysiology.

Icon Full text of the call for projects (PDF) (453.4 KB)

Icon Application form (Word) (199.0 KB)

You can also download these documents from the website of the Foundation for rare diseases (http://fondation-maladiesrares.org) by registering / logging in professional area, Call Projects section.

Research projects must be submitted via filled application form no later than Thursday, February 28, 2013:

We remain at your disposal for any further information and hope that you will respond to this call for many projects.
Wishing you every success in your projects.

 

Publication of results

The "Fondation maladies rares" and the National Infrastructure PHENOMIN are pleased to announce the results of the Call for Proposals 2013 for joint research projects "Murine models and rare diseases". The ad hoc Scientific Committee ("Fondation Maladies Rares" - PHENOMIN) has selected 20 projects amongst 60 submitted projects.

Congratulations to the laureates!

Icon List of selected projects (PDF) (61.0 KB)

1st Call - IMPC

Through its partnership in the IMPC, PHENOMIN will, over the next 5 years, produce over 300 transgenic mouse lines bearing a single gene knock-out and analyse them using broad-based, high-throughput phenotyping. This first call for proposal marks the beginning of this strong partnership.

These knockout mice, together with the wealth of associated data, will serve as a valuable, freely available, resource for French researchers.

Publication of results

249 genes were nominated by 85 French PI. Using two selection criterias (value of the scientific project and availability of at least 3 clones with conditional potential in the ES mutant cells resources (International Knockout Mouse Consortium - IKMC)), we were able to select 140 genes which enter the process of mouse model production and phenotyping.

Consequently, additional new mouse lines have been generated by PHENOMIN.

IMPC Alleles: IKMC - EUCOMM/KOMP

IMPC_alleles