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Mouse and Rat Cryopreservation

Cryopreservation offers several techniques to ensure the safety of your valuable research materials.

The benefits of cryopreservation includes:

  • Prevention of strain loss due to adventitious disease, pathogen contamination, genetic contamination, or any disaster inherent in live breeding colonies,
  • Preservation of your strain indefinitely,
  • Minimization of genetic drift within your strain,
  • Saving animal housing space, husbandry & labor costs,
  • Facilitating rederivation into barrier facilities,
  • Facilitating shipping, distribution, and exchange of your strains within the scientific community.

PHENOMIN has a proven and huge experience of collecting and freezing of large numbers of mouse germoplasm with more than 250 lines per year. Today, more than 2,000 mouse models are archived in PHENOMIN librairy ; a rat librairy is under development.

We offer both embryo and sperm cryopreservation for mice and embryo cryopreservation for rat strains. We also offer recovery services of your cryopreserved lines.

Mouse sperm & embryo cryopreservation

PHENOMIN’s Cryopreservation services using state-of-the-art technologies enables you to secure your valuable mice strains.

For Mouse, we offer both embryo and sperm cryopreservation. Sperm freezing is the most straightforward  technique to archive your strain. However, the choice of the technique depends on the characteristics of your strain. The below table will help you to make the most appropriate choice.

  Most appropriate for Pros Cons
Sperm
  • Single mutations on a common inbred derived strain (GEMs)
  • Small colonies
  • Less males needed
  • Work is done in one day
  • Inexpensive strain distribution (dry Ice for a 24h travel)
  • Give rise to large numbers of offspring following sperm resuscitation by in vitro fertilization (IVF)
  • Speed colony expansion from Sperm cryorecovery
  • Lower upfront cost
  • Cryopreserves half of genetic make-up (recovery of heterozygous)
  • Take more resources to get the desired genotype
Embryos
  • Homozygous
  • Multipled unliked mutations
  • or unique genetic background strain
  • You recover what you freeze
  • Fast cryorecovery
  • Can take a few months to get enough embryos stored
  • Requires females

 

Mouse Sperm Cryopreservation

Benefits
  • Get started quickly
  • Available for a variety of genetic backgrounds
  • Donor males may be shipped to PHENOMIN, whatever the health status
  • Ease the distribution of your strain
  • Set up rapidly a SPOF colony
Requirements
  • 4 males with at least 10 weeks of age
Deliverables 
  • At least 40 straws of sperm stored from a variety of strains and genetic backgrounds
  • In vitro quality control pre and post cryopreservation (including IVF rate and genotyping)
  • Blastocyst genotyping available upon request
  • In vivo quality control (recovery to live born) available upon request
  • Dual site storage

Mouse Embryo Cryopreservation

Benefits 
  • Best option to preserve complex genotypes and genetic backgrounds
  • Available for a variety of genetic backgrounds 
  • Donor animals may be shipped to PHENOMIN, whatever the health status 
  • Setting-up of SPOF colony
Requirements
  • At least 6 males needed (heterozygous or homozygous)
  • for homozygous embryos freezing (to recover the precise genotype), 6 to 10 homozygous FEmales in addition to homozygous males are required
Deliverables  
  • Cryopreservation of at least 200 embryos in 10 straws 
  • In vitro quality control pre and post cryopreservation   
  • In vivo quality control (recovery to live born) 
  • Dual site storage

Rat embryo cryopreservation

We provide secure and permanent prerservation of your rat colonies.

Benefits
  • Best option to minimize genetic drift and to prevent adventitious loss of your valuable strains
  • Available for a variety of genetic backgrounds
  • Donor animals may be shipped to PHENOMIN, whatever the health status
  • Setting-up of SPOF colony
Requirements
  • Only 6 males needed
Deliverables
  • Cryopreservation of at least 200 embryos in 10 straws 
  • In vitro quality control pre and post cryopreservation
  • In vivo quality control (recovery to live born)

CryoRecovery Services

We can produce live animals from cryopreserved germplasm whatever the source (PHENOMIN’s cryostock or outside sources). PHENOMIN has an extensive and proven experience of successfully recovering mouse & rat strains from frozen germplasm with an average of more than 150 strains per year.

Mouse Sperm Cryorecovery

Benefits
  • SOPF mice produced and shipped with a comprehensive health report
  • Larger cohorts of animals can be produced upon specific request
Requirements
  • 2 straws of frozen sperm (freezing protocol to be provided for sperm straws coming from other laboratories)
Deliverables
  • Recovery completed within weeks
  • At least 4 heterozygous mice with a defined health status
  • Genotyping available at additional cost

Mouse embryo Cryorecovery

Benefits
  • SOPF mice produced and shipped with a comprehensive health report
Requirements
  • 2 straws from PHENOMIN’s cryo-stock or 50-75 frozen embryos from other laboratories (Cryopreservation and Cryorecovery protocols to be provided)
Deliverables
  • Recovery completed within 8 to 12 weeks
  • At least 4 heterozygous mice with a defined health status
  • Genotyping available at additional cost

Rat embryo Cryorecovery

Requirements
  • 2 straws from PHENOMIN’s cryo-stock or 50-75 frozen embryos from other laboratories (Cryopreservation and Cryorecovery protocols to be provided)
Deliverables
  • Recovery completed within 12 weeks
  • At least 4 heterozygous rats with a defined health status
  • Genotyping available at additional cost