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New developments

Our R&D program

The PHENOMIN research and development program is clearly at the forefront of national and international projects for Genetic Engineering and Model Validation, Zootechny and Phenotyping. Each Work Package of this program is subdivided in sub-work packages with precisely identified deliverables, milestones and expected results allowing to establish straightforward criteria in order to evaluate the progression and the success of each Work Package.

These R&D programs have been developped in collaboration with external scientific experts to ensure that PHENOMIN is developing the most suitable resources and technologies for the community.

USERS / PARTNERS .
WP1: Infrastructure management
Databases and applications development
. USERS / PARTNERS
         
 
WP2
Mutagenesis
     
 
  • Development of innovative and pertinent tools for genetic engineering
  • Provide access to a high number of genetically modified mouse models
WP3
Zootechny
   
 
  • Implementation of methodologies and upgrading of facilities
  • Securing animal welfare
  • Ethic
WP4
Phenotyping
 
 
  • Development of new in vivo and in vitro functional analysis for mouse traits characterization
 
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WP5
Discover the function of unannotated genes and new translational mouse models
 

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Last developments achieved

Electroporation for genome editing in vivo

Electroporation 2019.png

We have implemented electroporation for the delivery of CRISPR/Cas9 with or without small donor DNA. We therefore completely respond to the principles of 3Rs (Replacement, Reduction and Refinement).

This method allows the drastic reduction of the number of donor females and fertilized oocytes. We consistently obtained high efficiency mouse genome editing for multiple genes, and we successfully generated mouse embryos with a variety of editing schemes, including indel mutations, point mutations, genomic deletions, and small precise insertions. Taken together, CRISPR-electroporation is a simple, economic, 3Rs friendly, high throughput, and highly efficient technique for genome editing in vivo, which can replace the traditional microinjection-dependent genome editing technique in mice and in other mammalian species.

CRISMERE technology

PHENOMIN developed the CRISPR Mediated REarrangement (CRISMERE) strategy to generate any kind of chromosomal rearrangement to generate trisomy 21 mouse and rat models. Have a look at our Personalized genome engineering page dedicated to this technology and read the recent publication in Scientific Report.

 Target DNA
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Inversion
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Duplication
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Deletion
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Integrated approach for bone pathology

We have developped new capabilities for analysing bone dynamics including both in vivo and ex vivo analysis of trabecular and cortical bone structures by microCT. This improves our current capacities of skeletal examinations by TRAP histological stain, X-ray, DEXA scanning, and quantitative NMR.

Contact us for help in designing your experiments on bone dynamics!

Metabo_Bone analysis