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Latest News

April 15, 2021 RESEARCH SPOTLIGHT

A resource of targeted mutant mouse lines for 5,061 genes (Nat Genet. 2021)

This April, an article in Nature Genetics highlights the major contribution of PHENOMIN-ICS in the International Mouse Phenotyping Consortium (IMPC) for the implementation of a comprehensive quality control strategy for alleles in targeted embryonic stem cell clones produced by high throughput. More than 5000 resulting mouse lines were generated with these ES cell clone through a collaborative effort of the different IMPC centers. All of them have the great advantage of having been established in the same C57BL/6N genetic background. Almost 3,700 have a versatile allele that has the advantage of being able to be transformed either into a conditional knockout or into a knockout bearing a LacZ reporter gene. The conditional allele remains a tool of choice for all scientists wishing to perform more detailed analyzes. The total number of genes with a potential conditional allele has been nearly doubled thanks to the effort of the IMPC.

The ultimate goal of IMPC is to generate and phenotype in a standardized manner the KOs of all protein-coding genes to provide key information for the biology and understanding of the development of human diseases. We were able to show that 72% of the KO lines show at least one phenotype, thus revealing a significant pleiotropy. This proportion includes the 39 % showing partial or total lethality. Thanks to this tremendous effort, almost half of the genome is now targeted. Since 2015, the production of KOs from ES cells has been replaced by simple KOs obtained by CRISPR / Cas9 technology. However, in the new era of genome editing, this ES cell-derived collection remains of unique value, as it offers particularly sophisticated and quality-controlled alleles representing a cornerstone of the collective development of a resource of null allele for the complete genome of mammals.

Marie- Christine Birling et al., 2021 - Nat Genet.

 

 

 


March 9, 2021 FREE LEARNING EVENTS

Health and safety of your model colonies: webinar event !

Do you want to take advantage of our expertise to manage the health and safety of your favorite models and to improve data reproducibility ?  Take the “fast track” towards protecting the health and safety of your model colonies with the Charles River's upcoming “Quick Insights in Animal Health” event in which PHENOMIN-ICS will share crucial information through the various presentations of track  3 and 6. The free learning series features short, on-demand presentations (each no more than 15 minutes) across six different tracks.

See you online, March 29th !

 


Feb. 25, 2021 NEWS

A Commitment to Transparency and Openness for Animal Research in France

The French interprofessional research think tank Gircor has just published a Transparency Charter on the use of animals for scientific and regulatory purposes signed by several entities of the scientific community in France, including PHENOMIN.

PHENOMIN is committed to ensuring better transparency by informing on current research and by giving the opportunity to understand the place of the animal models in scientific and biomedical research's progresses, and finally by explaining the conditions under which animal research is practiced.

The French initiative will strengthen the overall vision shared with many European countries and many scientists to support transparency for animal research.

Download


Feb. 9, 2021 JOB OPPORTUNITY

The PHENOMIN- ICS centre is recruiting- Come and join us !

IT Development Manager M/F - CDI

Reference: IGBMC / IT-16

IGBMC, one of Europe's leading biomedical research centers counting 700 employees of 45 nationalities and 47 research teams focusing on the study of human pathologies such as cancer and genetic diseases. The Mouse Clinical Institute (Institut Clinique de la Souris - iCS), the IGBMC's largest technology platform, is ISO 9001 and NFX 50-900 certified. It is the leading center of the national infrastructures PHENOMIN and CELPHEDIA and a key player in the European and global effort to annotate the mouse genome via the INFRAFRONTIER and IMPC consortia.

The IGBMC campus is located on the Illkirch Innovation Park in the suburbs of Strasbourg, France, an exceptional academic and industrial scientific environment that strongly favors collaborations and technology transfer.

For its activities the iCS relies on its IT Development Manager (M/F).

A permanent position to be filled as soon as possible

See the offer

Download


Jan. 18, 2021 RESEARCH SPOTLIGHT

Investigation of the role of Macrophages in the integrity and function of the colon epithelium in homeostasis

Maintenance of the intestinal barrier is critical given that it controls the absorption of nutrients, electrolytes, and water from the gut lumen into the blood circulation and further prevents the absorption of toxicluminal substances. Macrophages contribute not only to eliminating pathogens but also to the good functioning of most tissues of the human body (management of dead cells, healing, and maintenance of cardiac functions, arterial and neuronal). PHENOMIN-CIPHE has developed an innovative model of knock-in mice which has been used to induce Macrophages depletion (CD64DTR model). The research teams from the Institut Curie (Paris, France) used it to study role of Macrophages in the integrity and function of the colon epithelium in homeostasis. Authors show that sub-epithelial Macrophages perform a rapid quality check of the fluids absorbed through distal colon epithelial cells, protecting them from being poisoned by luminal fungal metabolites.

Chikina A S et al, 2020 –Cell


Dec. 23, 2020 OPEN CALL

15th Call for proposal - INFRAFRONTIER

COVID-19 research with mouse disease models

We are excited to inform you about INFRAFRONTIER´s new free-of-charge TA Call: COVID-19 Therapeutics Pipeline. Our goal is to provide extensive user support to the selected projects for assessing novel potential vaccine and treatment candidates for COVID-19. The TA service is covered by the INFRAFRONTIER2020 project (Horizon 2020). 

INFRAFRONTIER partners (PHENOMIN-CIPHE and CCP) will execute this call by providing viral particles, COVID-19 mouse models, cohort production, and BSL3 pipeline analysis. Further functional analysis and advanced readouts like organ histopathological analysis, multiplex assay profiling of cytokines and chemokines in lung and mouse serum, etc. can be provided on a collaborative basis outside the TA call scope. 

  • Application form: Please fill in the application form and send it to proposals@infrafrontier.eu
  • Application deadline: 31 January 2021
  • Application costs: This TA Call is free-of-charge for the selected applicants.
  • Please contact info@infrafrontier.eu if you have any questions.

For more information about this project and about how to apply please visit our website

For further questions please write to us: proposals@infrafrontier.eu

 


Dec. 17, 2020 ANNOUNCE

Online introduction to Mouse embryology (conducted in French)

PHENOMIN-ICS supports you in acquiring a general knowledge of the major stages of the mouse embryonic development; additionaly O.Wendling and H. Jacobs will train you to be able to design a first-line phenotyping experiment on mouse embryos, to determine a window of lethality in utero and finally to improve your knowledge of imaging techniques adapted to mouse embryos

The training  is scheduled online, next March 30 - 31st, and will be conducted in French.

More information


Oct. 26, 2020 RESOURCES

A customizable poster for your animal welfare body

The AWB is a regulatory structure under the European Directive 2010/63/EU and the French decree 2013-118 (Art. R. 214-103 of the Rural Code), mandatory for each breeder, supplier or user of animals for scientific purposes.
cus
CELPHEDIA network offers a customizable poster whose aim is to promote the missions of the AWB in France, to bring together local AWBs and their users. The poster can be downloaded in powerpoint format for customizing on request to celphedia@igbmc.fr. It is intended to be displayed in each animal facility to help all staff, especially newcomers, to understand what is the AWB. An update with your own AWB information is recommended for display in locations frequented by all concerned. The poster is available in French and in English, both versions being integrated into the file.

Download


Oct. 7, 2020 ONLINE WEBINAR

Mouse Study Reproducibility: the Impact of Genetic Background and Genotyping

In collaboration with our partner Charles River, Sylvie Jacquot (PHENOMIN-ICS) will present the impact of Genotyping in the mouse study reproducibility throught online webinar scheduled next week, on Thursday, October 15, 2020, 10:00 AM - 11:00 AM EDT (4:00 PM - 5:00 PM CEST)

Many researchers would agree that the extreme complexity of biological systems we study are a key factor impacting research reproducibility. For studies employing transgenic rodent lines, a lack of understanding of genetic backgrounds, as well as genetic drift, genetic contamination, and genotyping inconsistencies, all contribute to the issue of reproducibility in mouse studies.

Join us as we cover the following topics in this presentation:

  • Key factors impacting genetic background and characterization
  • Common inconsistencies/errors in genotyping and employing the latest tools/technology
  • Best practices for establishing rapid and reliable testing protocols

Registration


Sept. 10, 2020 INVITATION

Targeting Cancer with Animal Models - Move online !

 Mark your calendar for our upcoming annual INFRAFRONTIER 2020 Conference, which will focus on Targeting Cancer with Animal Models.

Please note: Due to the COVID-19 pandemic, the conference has been rescheduled to 07 - 08 October and moved online completely. 

Registration Deadline: 2. October! 

Registration: https://www.infrafrontier.eu/meeting-tool/xhtml/infraFrontierEvents.jsf

Event Page:  https://www.infrafrontier.eu/infrafrontier-research-infrastructure/public-relations/infrafrontier-conference-2020



Upcoming events

SYMPOSIUM

10th Annual Meeting of the EFOR network

May 10 - 11, 2021, Online

EFOR, French Network supporting the development of animal and plant species models is pleased to announce the 10th edition of the EFOR meeting, virtual event shceduled on May 10th and 11th ! It is a nice opportunity to understand more the interest of non common models which can be used to answer scientific question and then allow researcher to apply in the same way the  to implemente the 3Rs in practice. But not only ! The first day sessions focuses on scientific and technical topics reagrding mouse models, non-human primates models, fish models and organoids. And the the second day sessions focus on a particular model organism (Fish, plant, algae, ...)

Let's have a look to the scheduled program ! The registration to the meeting is free, and registration mandatory.  PHENOMIN will take part of the event.

Delighted to see you online !

More information at https://efor.fr/en/home/


WORKSHOP

11th Workshop on Innovative Mouse Models

June 3 - 24, 2021, Online

You are invited to register to the 11th Workshop on Innovative Mouse Models, organized by our colleagues from the Netherlands Cancer Institute (NKI). Because of the pandemic situation the workshop will be held ON-LINE and distributed over 4 seminar, in four Thursday Zoom sessions on June 3, 10, 17 and 24. The sessions include the folowing topics:

  • New CRISPR/Cas technologies
  • Relevance of disease models
  • Models for viral infection
  • Ethics and alternatives

Registration is free . Deadline Registration : June 1, 2021

 

More information at http://www.immworkshop.nl/

Click here to download the attachment.


TRAINING COURSE

Cell culture contamination (conducted in French)

June 8, 2021, Strasbourg, France

Objectives:

  • Become aware of the importance of traceability and quality control in cell culture
  • Understanding the issue of cell culture contamination
  • Become aware of the diversity of mycoplasma detection methods and be able to choose the techniques adapted to each case
  • Know how to prevent contamination and what attitudes to adopt in the event of proven contamination

The course is conducted in French- Acces to program and registration 

The training will be held online, for 2 consecutive mornings on June 7th and June 8th, if sanitary conditions require it.

CNRS formation entreprises

PHENOMIN's contact: bedu@igbmc.fr and wattenho@igbmc.fr (scientific director of the course)

More information at https://cnrsformation.cnrs.fr/contaminations-dans-cultures-cellulaires?mc=Contamination


TRAINING COURSE

Introduction to mouse embryology: theorical and practical aspects

June 22 - 23, 2021, Online

Objectives:

  • Acquire a general knowledge of the major stages of the mouse embryonic development
  • Be able to design a first-line phenotyping experiment on mouse embryos
  • Be able to determine a window of lethality in utero
  • Improving knowledge of imaging techniques adapted to mouse embryos

Program: 

Lectures - theoretical aspects (3 hrs)

  • Introduction to the embryonic development of mice: segmentation, gastrulation, the embryonic and foetal periods
  • Development of embryonic lineages

Workshops - practical aspects (3 hrs)

  • Determining the window of lethality of mutant mice in utero
  • Practical aspects of the collection and fixation of mouse embryos and placentas
  • Imaging techniques (HREM, MicroCT, OPT) of embryos

Organisation:

  • Online session - 2 consecutive mornings between June 22nd to June 23rd (whatever the sanitary conditions)
  • The session is conducted in French
  • Registration: online

CNRS formation entreprises

PHENOMIN's contact: bedu@igbmc.fr and olivia@igbmc.fr (scientific director of the course)

This theoretical training may be supplemented by face-to-face training including practical work in Nov. 2021

More information at https://cnrsformation.cnrs.fr/introduction-embryologie-souris?mc=21423%20Introduction


TRAINING COURSE

3rd Imaging PATHBIO master class

September 27 - October 5, 2021, Online

Objectives:

  • The master class aims to provide graduate, master, PhD and postdoc students with basic and expert knowledge to phenotype morphologically mouse models of human diseases.
  • It's a unique chance to understand the basic principles of several key methods from structural to functional imaging and to get an overview of the best practices including the 3’R rules to design scientific experiments.

Informations:

More information at https://imaging-pathbio.sciencesconf.org/


TRAINING COURSE

In vivo CRISPR-Cas9 genome editing

October 7 - 8, 2021, Strasbourg, France

More information at https://cnrsformation.cnrs.fr/in-vivo-crispr-cas9-genome-editing?mc=CRISPR


TRAINING COURSE

Mouse embryology: practical training course (conducted in French)

November 16 - 18, 2021, Strasbourg, France

Objectives

  • Acquire primary expertise in mouse development
  • Be able to design primary phenotyping experiment on mouse embryos
  • Learn about all the primary phenotyping analysis on mouse embryos
  • Be able to dissect post-implanted embryos
  • Be able to determine the window of lethality in utero
  • Be able to evaluate embryos viability at neonatal stage
  • Increase awareness of relevant technics to image embryos

Organisation:

Acces to detailed program and registration

CNRS formation entreprises

PHENOMIN's contact: bedu@igbmc.fr and olivia@igbmc.fr (scientific director of the course)

More information at https://cnrsformation.cnrs.fr/embryologie-souris?mc=embryo


MEETING

AFSTAL 2021

November 30 - December 2, 2021, Marseille, France

Postponed: 2021 Nov. 30th to Dec. 02nd

Attend the French congress in animal science

Les Sciences de l’Animal de Laboratoire tous les ans les scientifiques du domaine autour de thèmes scientifiques, règlementaires, éthiques et techniques. Le 46e Colloque annuel de l'association française se déroulera du 30 Novembre au 02 décembre 2021 dans la ville de Marseille et dont le titre sera “Expérimenter c’est aussi anticiper”. En effet, cette année L'AFSTAL traitera le thème de l’anticipation en vue de la réalisation d’un projet dans son ensemble : éthique, expérimental et scientifique et dediera la première session du colloque à la place de l’animal dans les progrès de la recherche pour répondre à une attente sociétale.

PHENOMIN sera présent à cet incontournable rendez vous français en Sciences de l’Animal de Laboratoire. venez nous rejoindre et nous rencontrer !

 

More information at https://www.colloque-afstal.com/2020/


TRAINING COURSE

WEBINAR Series: Mouse Study Reproducibility

December 4, 2021, Online

The complexity of biological systems we study are a key factor impacting research reproducibility. For studies employing transgenic rodent lines, a lack of understanding of genetic backgrounds, as well as genetic drift, genetic contamination, and genotyping inconsistencies, all contribute to the issue of reproducibility in mouse studies. For further insight we are pleased to present the following Charles River-hosted webinar presented by S. Jacquot  from PHENOMIN-ICS

PHENOMIN-ICS covers the following topics in this presentation

  1. Key factors impacting genetic background and characterization
  2. Common inconsistencies/errors in genotyping and employing the latest tools/technology
  3. Best practices for establishing rapid and reliable testing protocols

Genotyping step is crucial in the model creation process as it allows to select animals carrying the good combination of alleles for phenotyping analyses. The different technics are based on the search for a variation in the DNA sequence (in length, composition, quantification...) located at a well-defined place in the genome. PHENOMIN-ICS offers customized genotyping services for knock-out, knock-in, transgenic or any other genetically modified murine model. Different sample types can be processed, including tail biopsies, ear punches, embryonic yolk sac, as well as non-invasive biopsies. We provide a detailed genotyping protocol to all our users on request. 

 How can we help you? Request a quote or email us.

More information at http://www.phenomin.fr/en-us/contact/


TRAINING COURSE

WEBINAR Series: Transgenic Mouse and Rat Model Creation

December 5 - January 5, 2021, Online

CRISPR-Cas9 is a rapid and cost effective technology to generate mouse and rat mutant models, however, its impact on the genome still requires careful consideration. For further insight we are pleased to present the following Charles River-hosted and Labroots-hosted webinars presented by G. Pavlovic and M-C Birling from PHENOMIN-ICS

PHENOMIN-ICS works with you from concept to model creation using homologus recombination, latest CRISPR/Cas9 and RNAi technology, and either other trangenic technics to deliver the most relevant model for your application. The effective research with rodent models depends on finding the best appropriated model for your project. How can we help you? Request a quote or email us.

More information at http://www.phenomin.fr/en-us/contact/


News

April 15, 2021
A resource of targeted mutant mouse lines for 5,061 genes (Nat Genet. 2021)
March 9, 2021
Health and safety of your model colonies: webinar event !
Feb. 25, 2021
A Commitment to Transparency and Openness for Animal Research in France
Feb. 9, 2021
The PHENOMIN- ICS centre is recruiting- Come and join us !
Jan. 18, 2021
Investigation of the role of Macrophages in the integrity and function of the colon epithelium in homeostasis
Dec. 23, 2020
15th Call for proposal - INFRAFRONTIER
Dec. 17, 2020
Online introduction to Mouse embryology (conducted in French)
Oct. 26, 2020
A customizable poster for your animal welfare body
Oct. 7, 2020
Mouse Study Reproducibility: the Impact of Genetic Background and Genotyping
Sept. 10, 2020
Targeting Cancer with Animal Models - Move online !

Events

May 10 - 11, 2021, Online
10th Annual Meeting of the EFOR network
June 3 - 24, 2021, Online
11th Workshop on Innovative Mouse Models
June 8, 2021, Strasbourg, France
Cell culture contamination (conducted in French)
June 22 - 23, 2021, Online
Introduction to mouse embryology: theorical and practical aspects
September 27 - October 5, 2021, Online
3rd Imaging PATHBIO master class
October 7 - 8, 2021, Strasbourg, France
In vivo CRISPR-Cas9 genome editing
November 16 - 18, 2021, Strasbourg, France
Mouse embryology: practical training course (conducted in French)
November 30 - December 2, 2021, Marseille, France
AFSTAL 2021
December 4, 2021, Online
WEBINAR Series: Mouse Study Reproducibility
December 5 - January 5, 2021, Online
WEBINAR Series: Transgenic Mouse and Rat Model Creation