Latest News

Feb. 6, 2018 NOW

PHENOMIN has developed expertise for oncology studies

PHENOMIN has developed expertise for oncology studies using mouse models for cancer progression and/or initiation. Some of our scientists is attending the Toulouse onco Week  2018 and present over the poster session. They are available to discuss projects in close collaboration with you. 


Jan. 25, 2018 COURSES

One week left to apply to the 3rd Programmable Nucleases (CRISPR/Cas9) Transgenesis Course

Applications for the 3rd Programmable Nucleases (CRISPR/Cas9) Transgenesis Course hosted by Czech Centre for Phenogenomics  - Institute of Molecular Genetics ASCR in Prague with the collaboration of PHENOMIN are still opened.

Dead line: January 31st 2018

This course is designed to give practical, hands-on training in current technologies and methods employing programmable nucleases (CRISPR/Cas9) -mediated genome manipulations in the generation of mice mutants. Participants will gain theoretical and practical knowledge from our expert instructors and have the opportunity for discussion during our evening social events including the opportunity for expert insight for your own individual project.


Dec. 18, 2017 PARTNERSHIP

AXENIS / PHENOMIN-CIPHE partnership: integrated know-how

The October the 4th 2017, the Inserm’s infrastructure division highlighted the activities of the Center for Immunophenomics (PHENOMIN-CIPHE, a joint Inserm service unit with CNRS and Aix-Marseille UMS 12 University) based in Marseille and headed by Bernard Malissen and the AXENIS-spin off services company of Institut Pasteur and expert in the development of humanized murine models for preclinical trials in immunology and oncology headed by Erwan Corcuff. In the presence of the Director General of INSERM Yves Lévy, the partnership between CIPHE and AXENIS was officially signed.

The integrated know-how resulting from the AXENIS-CIPHE partnership makes it possible to extensively characterize the immune system of such mice and to use this knowledge in the development of effective vaccines, pathogenic drugs and human tumors.

The scientific community is invited to clarify their specific needs for preclinical models on the measurement of human diseases in the areas of inflammation, infection, immuno-oncology and immunization, as well as their functional exploration including the use of humanized murine models highly predictive of human responses.

This partnership will contribute to advances in immunotherapy in the field of public and private research.

Please contact  PHENOMIN-CIPHE experts to describe your needs regarding innovative murine models applied to infectiology and immuno-oncology

On line videos


Dec. 12, 2017 NEWS

Blastocyst genotyping- A new approach to limit the number of animals used in research.

The 3R* (Replace, Reduce, Refine) principle is the guiding principle for more ethical use of animals in research.

Respecting this principle is a legal obligation throughout the European Union and in more and more countries wordwide. It guarantees that the animals are used only: if there is no other method, in minimum number and with the best practices to guaranty the well-being of the animals. Concretely, many actions have been taken around the world and in our institute (lien ici page ethique) to apply this principle.

In September 2017, our blastocyst genotyping publication « Blastocyst genotyping for quality control of mouse mutant archives: an ethical and economical approach » was nominated, by the management team of INFRAFRONTIER, for a “global 3R's award” (upload document) from AAALAC. PHENOMIN was involved in the setting up of this alternative to reduce the number of animals used and improve the quality control to determine the ability of a mutant mouse line to recover after cryopreservation. It’s now routinely used in PHENOMIN projects. Many thanks for this nomination! Congratulations to the 2017 Global 3Rs Award Winners!

* This 3R principle is also referred to as "alternative methods" in the regulations. This expression may be misunderstanding. For the non-scientific, an alternative method should be a method that does not use live animals, which is only one aspect of the 3Rs that include also reduction and refinement.


Nov. 23, 2017 SPECIAL EVENT

Meet us at the BioFIT- 2017 !

Are you ready for innovations ? If so, PHENOMIN-ICS welcomes you for meeting and discussion at the BioFIT- 2017, leading partnering event in Europe for technology transfer, academia-industry collaborations and early-stage innovations in the field of Life Sciences. 

Join and meet us at the booth E2/D8-5 ! 


Nov. 22, 2017 CALL

Precision mammalian model development

INFRAFRONTIER2020 Project - Trans-national Access call - November 2017
Precision mammalian model development
 

Context and aim of the call
INFRAFRONTIER is the European Research Infrastructure for phenotyping, archiving and distribution of model mammalian genomes. It provides access to first-class tools and data for biomedical research, and thereby contributes to improving the understanding of gene function in human health and disease using the mouse model. It facilitates access for the wider biomedical research community to deliver novel mouse lines that will advance knowledge of human disease and will be of widespread use in biomedical science. 

During the course of the INFRAFRONTIER2020 project a total of 3 Trans-national Access calls for the development of mammalian model development supporting a total of 28 projects will be published. Further calls supporting 10 mouse model and 6 rat model development projects will be released in 2018.

More information and call application form: https://www.infrafrontier.eu/resources-and-services/infrafrontier-open-calls/precision-mammalian-model-development

Proposal submission to proposals@infrafrontier.eu by 20 December 2017.
Proposal evaluation from 21 December 2017 to 15 February 2018.

The INFRAFRONTIER2020 project has received funding from the EU Research and Innovation programme Horizon 2020 (H2020-EU.1.4.1.1. Developing new world class research infrastructures)


Nov. 16, 2017 SERVICES

PHENOMIN's expertise for your oncology studies

PHENOMIN has developed expertise for oncology studies using mouse models for cancer progression and/or initiation. We are available to discuss projects, in close collaboration with users in order to customize any study to the scientific objectives applying the best adhesion to 3R rules. Our services:

  • Progression and initiation models 
  • Bioluminescent tumor cells
  • Phenotyping and immunophenotyping

Have a look to our whole offer !


Nov. 9, 2017 WEBINAR

Using Cre-lox Recombination to Discover Gene Functions

PHENOMIN-ICS will present a webinar "The Cre-Toolbox: Revealing Gene Functions with Cre-lox Recombination", November 14, 2017, 9:00 AM EST (3:00 PM CET)

this webinar will discuss

  • Using Cre-lox to understand pleiotropy and dissecting gene functions
  • Overcoming embryonic and early postnatal lethality
  • Inducible inactivation with the creERT2 system
  • Phenotypes induced by Cre driver, tamoxifen toxicity, Cre leakiness: What are the unexpected complexities and technical limitations?
  • CRISPR/Cas9 genome editing for in vivo spatial and/or temporal gene inactivation

Presenters

  • Guillaume Pavlovic, PhD, Head of the Genetic Engineering and Model Validation Department, PHENOMIN-ICS

Register at: http://www2.criver.com/l/60962/2017-10-24/f9rj5b

CreERT2 repository for the collection and distribution of relevant mutant mouse strains: http://mousecre.phenomin.fr/   



Upcoming events

WORKSHOP

Advanced data analysis Workshop

March 6, 2018, Nantes, France

The french technical plateforme Cytocell organizes a workshop "advanced data analysis methodologies for multi parameter cytometry datasets". In the morning, an ovewiew of all the technical panels use to analyse, and in the afternoon, a workshop focusiong on one tehcnics you decide to deeper understand.

Cytocell belongs to the Federative Research Structure (FRS) François Bonamy (FED 4203 / Inserm UMS 016/CNRS 3556) which brings together the research units and laboratories of the local health cluster around a common scientific strategy and consists in all the research teams within SFR belong to the faculties of medicine, pharmacy, dentistry and science, managing 19 plateforms. 

Information and registration : juliette.desfrancois@univ-nantes.fr  

More information at http://www.sfrsante.univ-nantes.fr/accueil/evenements-pour-la-recherche/plate-forme-cytocell-ournee-analyse-de-datas-advanced-avec-analyse-non-supervisee-analyse-en-clustering--216


TRAINING COURSE

In vivo CRISPR-Cas9 genome editing

March 14 - 15, 2018, Illkirch, France

Objectives

PHENOMIN's training aims to provide a general framework to get scientists started using CRISPR/Cas9 for in vivo gene editing in rodents.

The main topics are:

  • Learn more about gene editing and how it works
  • Hear about current advances on many technical aspects
  • Optimize the RNA guide design to the genotyping analysis (bioinformatics workshop).
  • Highlight crucial issue in your own scientific project.

The course in conducted in English.

One other session shceduled in November 2018.

Acces to Detailed program and registration procedure

CNRS formation entreprise

PHENOMIN's contact:
bedu@igbmc.fr

More information at https://cnrsformation.cnrs.fr/stage-18188-In-vivo-CRISPR-Cas9-genome-editing.html?stage=18188&mc=mouse


SYMPOSIUM

French JT Mass Cytometry

March 21, 2018, Paris, France

The French CyTOF group is happy to invite all students and scientists interested in cytometry for a second opus and discover what Mass Cytometry can brings to your research.

The broad field of application of mass cytometry is highlighted by a selection of original presentations in the field of myology, hematology, cancer and immunology and will also address a new area with Imaging Mass Cytometry.

The day will close with a round table discussion on the technology to help users apply faster mass cytometry protocols in their research.

The Organizing and the Scientific Committees

Thibault Andrieu
, Benoit Beitz
, Catherine Blanc, Aurélien Corneau, Antonio Cosma
, Hélène Gary, Émilie Grégori
, Coralie Guerin
, Hervé Luche
, Olivier Molendi-Coste, Laurent Pineau
, Fabrice Porcheray et Maryline Ripaux-Lefevre

E. Grecori, from PHENOMIN-CIPHE, will present relevant news in using mass cytometry.

More information at https://www.alphavisa.com/jtafc/2018/03/


TRAINING COURSE

Primary phenotyping of mouse embryos

March 27 - 29, 2018, Illkirch, France

Objectives

This training is aimed at providing a theoretical and practical background knowledge destined for researchers and engineers that are willing to acquire primary expertise in mouse development. The topics will cover dissection of post implant embryos, histology, whole mount Lac Z staining and confocal imaging of whole embryos.

Prospectives attendees

Researchers, engineers, scientists. Need to have the basic knowledge in animal biology to understand mouse development physiology and the biological technics for phenotyping. 

Content

Lectures 

  • Phenotyping of mouse embryos 
  • Determining the window of lethality of mutant mice in utero
  • Introduction to Mouse embryonic development
  • Classical histology (Fixation, embedding, orientation, sectioning and staining),
  • Immunohistochemistry and in situ hybridization
  • Imaging technics (HREM, micro CT, OPT)
  • High frequency echography to visualize and quantify embryonic development
  • The in-vitro culture of mouse embryos/ drug testing
  • Confocal macroscopy: application in embryology


Workshops

  • Collection and fixation of mouse embryos and placentas at E10.5 (mid gestation stage)
  • Whole mount Lac Z staining of E10.5 embryos (Step1)
  • Observation of embryos by echography
  • Observation of embryos by micro CT, OPT (potentialy)
  • Whole mount Lac Z staining of E10.5 embryos (step 2) + observation
  • Collection of mouse embryos at E8.5 (early embryonic stage)
  • Collection of mouse fetuses and placentas at E18.5 (just before birth)
  • Observation of embryos at the confocal macroscope
  • Visualization of histological sections at different stages of development. Example of abnormalities.

More information at https://sfc.unistra.fr/formations/chimie-bio_-_experimentation-animale-primatologie_-_primary-phenotyping-of-mouse-embryos_-_1162/


TRAINING COURSE

DCBIOL course - Biology of dendritic cells

March 27 - 28, 2018, Marseille, France

This course is organized by the labex DCBIOL, https://science.curie.fr/recherche/labex-dcbiol/, which gathers eight teams from the Institut Curie in Paris and three from the Center of Immunology of Marseille-Luminy, and focuses on the study of the biology of dendritic cells.

The course will be held in Marseille, at the Centre d'Immunologie Marseille-Luminy (CIML). How to access CIML : http://www.ciml.univ-mrs.fr/guide/access

REGISTRATION is free but mandatory. The number of seats is limited.

Coffee breaks and lunches are included. Please fill the form below to register. Deadline for registration is February 28th 2018. Contact: course@labex-dcbiol.fr

More information at http://labex-dcbiol.fr/


WORKSHOP

ISAC Cytometry Workshop

April 9 - 13, 2018, Paris, France

The 1st Paris ISAC Cytometry Workshop is jointly organized by the Institut Curie and the Institut Pasteur in collaboration with ISAC (International Society for the Advancement of Cytometry), AFC (Association Française de Cytométrie), and PHENOMIN-CIPHE (Centre d’immunophénomique, Marseille). The workshop will cover basic and advanced cytometry topics and will consist of lectures and practical modules taught by international experts in the field.

This course will offer a wide range of cytometry applications at all levels, Beginners will learn the basics of flow cytometry, how a cytometer works, fluorochromes, spillover and compensation, and commonly used techniques including proliferation, cell cycle, apoptosis and imaging flow cytometry.

Early bird registration: 15 February 2018  On line registration 

PHENOMIN's contact:

herve.luche@inserm.fr

More information at https://www.alphavisa.com/cytometry-workshop/2018/index.php


TRAINING COURSE

3rd Programmable Nucleases (CRISPR/Cas9) Transgenesis Course

April 16 - 20, 2018, Prague-Vestec, Czech

Applications for the 3rd Programmable Nucleases (CRISPR/Cas9) Transgenesis Course hosted by Czech Centre for Phenogenomics  - Institute of Molecular Genetics ASCR in Prague with the collaboration of PHENOMIN are still opened.

This course is designed to give practical, hands-on training in current technologies and methods employing programmable nucleases (CRISPR/Cas9) -mediated genome manipulations in the generation of mice mutants. Participants will gain theoretical and practical knowledge from our expert instructors and have the opportunity for discussion during our evening social events including the opportunity for expert insight for your own individual project.

https://www.infrafrontier.eu/resources-and-services/training-and-consulting-services/model-development-training-courses

 

More information at https://www.phenogenomics.cz/2017/12/3rd-programmable-nucleases-crisprcas9-transgenesis-course/


WORKSHOP

44ième colloque AFSTAL 2018

June 13 - 15, 2018, Reims, France

Attend the French congress in animal laboratory sciences

Cette année, l'association a décidé de traiter le thème des “limites de l’expérimentation animale au sens large”.

"Au nom de l’AFSTAL, j’ai donc le plaisir de vous convier au 44e Colloque de notre Association, qui se tiendra du 13 au 15 juin 2018 dans la ville de Reims et dont le titre sera “Recherche, Modèle animal, Société”.

Dans la lignée des éditions précédentes, nous consacrerons la première session du colloque à la présentation des plateformes locales et des équipes scientifiques de la région. Nous consacrerons ensuite l’ensemble des conférences aux “limites” dans tous leurs états en portant un regard particulier sur l’intérêt du modèle animal, ses limites, sa dimension éthique en recherche, son rapport et sa perception par la société ainsi que sa réhabilitation. L’aspect scientifique, expérimental et fonctionnel du modèle animal sera ainsi abordé.

Nous apporterons encore cette année une attention toute particulière aux retours d’expérience. C’est pourquoi nous retrouverons au sein du programme de ce colloque, des communications libres et des ateliers de fournisseurs.

La plus grande exposition commerciale d’Europe sera accessible durant la totalité du colloque afin de favoriser les discussions et les échanges entre les congressistes et les exposants. En effet, tous les partenaires, fournisseurs, distributeurs de matériels et de services, incontournables dans le domaine des sciences de l’animal de laboratoire, seront au rendez-vous. À cette occasion, tout en participant au programme scientifique, vous pourrez découvrir les dernières nouveautés et développer votre réseau professionnel et personnel.

L’AFSTAL vous attend donc tous nombreux pour son 44e Colloque qui, comme chaque année, sera organisé de sorte à rendre ce moment aussi convivial que studieux.

Pour le Comité d’Organisation
Frédéric Jacquot, Président de l’AFSTAL"

More information at https://www.colloque-afstal.com/2018/