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This April, an article in Nature Genetics highlights the major contribution of PHENOMIN-ICS in the International Mouse Phenotyping Consortium (IMPC) for the implementation of a comprehensive quality control strategy for alleles in targeted embryonic stem cell clones produced by high throughput. More than 5000 resulting mouse lines were generated with these ES cell clone through a collaborative effort of the different IMPC centers. All of them have the great advantage of having been established in the same C57BL/6N genetic background. Almost 3,700 have a versatile allele that has the advantage of being able to be transformed either into a conditional knockout or into a knockout bearing a LacZ reporter gene. The conditional allele remains a tool of choice for all scientists wishing to perform more detailed analyzes. The total number of genes with a potential conditional allele has been nearly doubled thanks to the effort of the IMPC.
The ultimate goal of IMPC is to generate and phenotype in a standardized manner the KOs of all protein-coding genes to provide key information for the biology and understanding of the development of human diseases. We were able to show that 72% of the KO lines show at least one phenotype, thus revealing a significant pleiotropy. This proportion includes the 39 % showing partial or total lethality. Thanks to this tremendous effort, almost half of the genome is now targeted. Since 2015, the production of KOs from ES cells has been replaced by simple KOs obtained by CRISPR / Cas9 technology. However, in the new era of genome editing, this ES cell-derived collection remains of unique value, as it offers particularly sophisticated and quality-controlled alleles representing a cornerstone of the collective development of a resource of null allele for the complete genome of mammals.
Marie- Christine Birling et al., 2021 - Nat Genet.
Do you want to take advantage of our expertise to manage the health and safety of your favorite models and to improve data reproducibility ? Take the “fast track” towards protecting the health and safety of your model colonies with the Charles River's upcoming “Quick Insights in Animal Health” event in which PHENOMIN-ICS will share crucial information through the various presentations of track 3 and 6. The free learning series features short, on-demand presentations (each no more than 15 minutes) across six different tracks.
See you online, March 29th !
The French interprofessional research think tank Gircor has just published a Transparency Charter on the use of animals for scientific and regulatory purposes signed by several entities of the scientific community in France, including PHENOMIN.
PHENOMIN is committed to ensuring better transparency by informing on current research and by giving the opportunity to understand the place of the animal models in scientific and biomedical research's progresses, and finally by explaining the conditions under which animal research is practiced.
The French initiative will strengthen the overall vision shared with many European countries and many scientists to support transparency for animal research.
IGBMC, one of Europe's leading biomedical research centers counting 700 employees of 45 nationalities and 47 research teams focusing on the study of human pathologies such as cancer and genetic diseases. The Mouse Clinical Institute (Institut Clinique de la Souris - iCS), the IGBMC's largest technology platform, is ISO 9001 and NFX 50-900 certified. It is the leading center of the national infrastructures PHENOMIN and CELPHEDIA and a key player in the European and global effort to annotate the mouse genome via the INFRAFRONTIER and IMPC consortia.
The IGBMC campus is located on the Illkirch Innovation Park in the suburbs of Strasbourg, France, an exceptional academic and industrial scientific environment that strongly favors collaborations and technology transfer.
For its activities the iCS relies on its IT Development Manager (M/F).
A permanent position to be filled as soon as possible
Maintenance of the intestinal barrier is critical given that it controls the absorption of nutrients, electrolytes, and water from the gut lumen into the blood circulation and further prevents the absorption of toxicluminal substances. Macrophages contribute not only to eliminating pathogens but also to the good functioning of most tissues of the human body (management of dead cells, healing, and maintenance of cardiac functions, arterial and neuronal). PHENOMIN-CIPHE has developed an innovative model of knock-in mice which has been used to induce Macrophages depletion (CD64DTR model). The research teams from the Institut Curie (Paris, France) used it to study role of Macrophages in the integrity and function of the colon epithelium in homeostasis. Authors show that sub-epithelial Macrophages perform a rapid quality check of the fluids absorbed through distal colon epithelial cells, protecting them from being poisoned by luminal fungal metabolites.
COVID-19 research with mouse disease models
We are excited to inform you about INFRAFRONTIER´s new free-of-charge TA Call: COVID-19 Therapeutics Pipeline. Our goal is to provide extensive user support to the selected projects for assessing novel potential vaccine and treatment candidates for COVID-19. The TA service is covered by the INFRAFRONTIER2020 project (Horizon 2020).
INFRAFRONTIER partners (PHENOMIN-CIPHE and CCP) will execute this call by providing viral particles, COVID-19 mouse models, cohort production, and BSL3 pipeline analysis. Further functional analysis and advanced readouts like organ histopathological analysis, multiplex assay profiling of cytokines and chemokines in lung and mouse serum, etc. can be provided on a collaborative basis outside the TA call scope.
For more information about this project and about how to apply please visit our website
For further questions please write to us: proposals@infrafrontier.eu
PHENOMIN-ICS supports you in acquiring a general knowledge of the major stages of the mouse embryonic development; additionaly O.Wendling and H. Jacobs will train you to be able to design a first-line phenotyping experiment on mouse embryos, to determine a window of lethality in utero and finally to improve your knowledge of imaging techniques adapted to mouse embryos
The training is scheduled online, next March 30 - 31st, and will be conducted in French.
The AWB is a regulatory structure under the European Directive 2010/63/EU and the French decree 2013-118 (Art. R. 214-103 of the Rural Code), mandatory for each breeder, supplier or user of animals for scientific purposes.
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CELPHEDIA network offers a customizable poster whose aim is to promote the missions of the AWB in France, to bring together local AWBs and their users. The poster can be downloaded in powerpoint format for customizing on request to celphedia@igbmc.fr. It is intended to be displayed in each animal facility to help all staff, especially newcomers, to understand what is the AWB. An update with your own AWB information is recommended for display in locations frequented by all concerned. The poster is available in French and in English, both versions being integrated into the file.
In collaboration with our partner Charles River, Sylvie Jacquot (PHENOMIN-ICS) will present the impact of Genotyping in the mouse study reproducibility throught online webinar scheduled next week, on Thursday, October 15, 2020, 10:00 AM - 11:00 AM EDT (4:00 PM - 5:00 PM CEST)
Many researchers would agree that the extreme complexity of biological systems we study are a key factor impacting research reproducibility. For studies employing transgenic rodent lines, a lack of understanding of genetic backgrounds, as well as genetic drift, genetic contamination, and genotyping inconsistencies, all contribute to the issue of reproducibility in mouse studies.
Join us as we cover the following topics in this presentation:
Mark your calendar for our upcoming annual INFRAFRONTIER 2020 Conference, which will focus on Targeting Cancer with Animal Models.
Please note: Due to the COVID-19 pandemic, the conference has been rescheduled to 07 - 08 October and moved online completely.
Registration Deadline: 2. October!
Registration: https://www.infrafrontier.eu/meeting-tool/xhtml/infraFrontierEvents.jsf
EFOR, French Network supporting the development of animal and plant species models is pleased to announce the 10th edition of the EFOR meeting, virtual event shceduled on May 10th and 11th ! It is a nice opportunity to understand more the interest of non common models which can be used to answer scientific question and then allow researcher to apply in the same way the to implemente the 3Rs in practice. But not only ! The first day sessions focuses on scientific and technical topics reagrding mouse models, non-human primates models, fish models and organoids. And the the second day sessions focus on a particular model organism (Fish, plant, algae, ...)
Let's have a look to the scheduled program ! The registration to the meeting is free, and registration mandatory. PHENOMIN will take part of the event.
Delighted to see you online !
More information at https://efor.fr/en/home/
You are invited to register to the 11th Workshop on Innovative Mouse Models, organized by our colleagues from the Netherlands Cancer Institute (NKI). Because of the pandemic situation the workshop will be held ON-LINE and distributed over 4 seminar, in four Thursday Zoom sessions on June 3, 10, 17 and 24. The sessions include the folowing topics:
Registration is free . Deadline Registration : June 1, 2021
More information at http://www.immworkshop.nl/
Click here to download the attachment.
Objectives:
The course is conducted in French- Acces to program and registration
The training will be held online, for 2 consecutive mornings on June 7th and June 8th, if sanitary conditions require it.
CNRS formation entreprises
PHENOMIN's contact: bedu@igbmc.fr and wattenho@igbmc.fr (scientific director of the course)
More information at https://cnrsformation.cnrs.fr/contaminations-dans-cultures-cellulaires?mc=Contamination
Objectives:
Program:
Lectures - theoretical aspects (3 hrs)
Workshops - practical aspects (3 hrs)
Organisation:
PHENOMIN's contact: bedu@igbmc.fr and olivia@igbmc.fr (scientific director of the course)
This theoretical training may be supplemented by face-to-face training including practical work in Nov. 2021
More information at https://cnrsformation.cnrs.fr/introduction-embryologie-souris?mc=21423%20Introduction
Objectives:
Informations:
More information at https://imaging-pathbio.sciencesconf.org/
This training aims to provide a general framework to get scientists started using CRISPR-Cas9 for in vivo gene editing in rodents.
Objectives
Acces to program and registration
The training will be held online, for 3 consecutive mornings, on the same dates, if sanitary conditions require it.
CNRS formation entreprises
PHENOMIN's contact: bedu@igbmc.fr and birlingm@igbmc.fr (scientific director of the course)
More information at https://cnrsformation.cnrs.fr/in-vivo-crispr-cas9-genome-editing?mc=CRISPR
Objectives
Organisation:
Acces to detailed program and registration
CNRS formation entreprises
PHENOMIN's contact: bedu@igbmc.fr and olivia@igbmc.fr (scientific director of the course)
More information at https://cnrsformation.cnrs.fr/embryologie-souris?mc=embryo
Postponed: 2021 Nov. 30th to Dec. 02nd
Attend the French congress in animal science
Les Sciences de l’Animal de Laboratoire tous les ans les scientifiques du domaine autour de thèmes scientifiques, règlementaires, éthiques et techniques. Le 46e Colloque annuel de l'association française se déroulera du 30 Novembre au 02 décembre 2021 dans la ville de Marseille et dont le titre sera “Expérimenter c’est aussi anticiper”. En effet, cette année L'AFSTAL traitera le thème de l’anticipation en vue de la réalisation d’un projet dans son ensemble : éthique, expérimental et scientifique et dediera la première session du colloque à la place de l’animal dans les progrès de la recherche pour répondre à une attente sociétale.
PHENOMIN sera présent à cet incontournable rendez vous français en Sciences de l’Animal de Laboratoire. venez nous rejoindre et nous rencontrer !
More information at https://www.colloque-afstal.com/2020/
The complexity of biological systems we study are a key factor impacting research reproducibility. For studies employing transgenic rodent lines, a lack of understanding of genetic backgrounds, as well as genetic drift, genetic contamination, and genotyping inconsistencies, all contribute to the issue of reproducibility in mouse studies. For further insight we are pleased to present the following Charles River-hosted webinar presented by S. Jacquot from PHENOMIN-ICS
PHENOMIN-ICS covers the following topics in this presentation
Genotyping step is crucial in the model creation process as it allows to select animals carrying the good combination of alleles for phenotyping analyses. The different technics are based on the search for a variation in the DNA sequence (in length, composition, quantification...) located at a well-defined place in the genome. PHENOMIN-ICS offers customized genotyping services for knock-out, knock-in, transgenic or any other genetically modified murine model. Different sample types can be processed, including tail biopsies, ear punches, embryonic yolk sac, as well as non-invasive biopsies. We provide a detailed genotyping protocol to all our users on request.
How can we help you? Request a quote or email us.
More information at http://www.phenomin.fr/en-us/contact/
CRISPR-Cas9 is a rapid and cost effective technology to generate mouse and rat mutant models, however, its impact on the genome still requires careful consideration. For further insight we are pleased to present the following Charles River-hosted and Labroots-hosted webinars presented by G. Pavlovic and M-C Birling from PHENOMIN-ICS
PHENOMIN-ICS works with you from concept to model creation using homologus recombination, latest CRISPR/Cas9 and RNAi technology, and either other trangenic technics to deliver the most relevant model for your application. The effective research with rodent models depends on finding the best appropriated model for your project. How can we help you? Request a quote or email us.
More information at http://www.phenomin.fr/en-us/contact/
