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Immunoprofiling

We offer high-throughput solutions for immunity profiling and characterization of your model. This includes

For any scientific, technological or strategy guidance, please do not hesitate to contact PHENOMIN experts.

Our Immunity profiling workflow

 

Immuno_Souris

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Biosample collection

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Automated cell extraction

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experimental groups - up to 24 individuals
 
up to 4 tissues
 
up to 24 sampes at once
   
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EX VIVO ASSAYS

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Cell sorting (11 immune cell types)
High content panel
Metabolic probes
Surface & intracellular markers
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Transcriptomic analysis
Immunoprofiling
Single cell Functional analysis
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Multiparametric flow & Spectral cytometry
Mass cytometry
 

IN VIVO ASSAYS

Activation & Differentiation (B, T, NK cells)
Cytokine, Chemokine multiplex Immuno assays

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CELL BANKING

Basal & Activated states

INTEGRATED DATA ANALYSIS

Our high content Immune phenotyping panels

IMMUNE ORGANS
 
HIGH CONTENT PANELS
Blood
Thymus
Spleen / Lymphe Node
Bone marrow
Peritoneum
Lung
Intestin
 
PERIPHERAL BLOOD
Quantitative analysis of hematopoietic cells
 
13 populations
 
THYMUS
T cell development
Dendritic cells
 
17 populations
19 populations
 
SPLEEN / LYMPHE NODE
Orientation panel
L-T/NK panel
L-B panel
Myeloid panel
Dendritic panel
 
34 populations
53 populations
15 populations
19 populations
19 populations
CELL TYPES
LYMPHOCYTES
T cells
B cells
NK cells
 
MYELOID CELLS
Monocytes
Neutrophils/Basop
Eosinophils/Mast
Macrophages
DC, pDC
 
BONE MARROW
Stem cells / Myeloid cells precursors
B cell development

 

 
 
LUNG (broncho-alveolar lavage)
Steady state/ inflammation
 
17 populations
 
PERITONEAL CAVITY
Steady state/ inflammation
 
17 populations
 
Quantitative measurement of MARKER EXPRESSION
20 populations
   
DESIGN PANEL & CONSULTING
 

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Biosafety Level-3 lab (BSL3): a secure platform to work safetly with class II / III Pathogens

 

Tracking & characterizing immunity in infected mouse models

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Monitoring infection progression

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Delivery of key analysis endpoints


INFECTION MONITORING

Immuno_Souris

• CTRL
• GEM
• HUMANIZED
Acute & Chronic Infection
 
     

EX VIVO ASSAYS

PATHOGEN COLONIZATION
CFU
 
INNATE & ADAPTATIVE IMMUNITY
Cell sorting of infected cells
Immunophenotyping
Serum released cytokines & chemokines profiling
Immune cells stimulation with pathogen antigens
 
ANATOMOPATHOLOGY
Confocal imaging
Tissues cryosection
Histology H&E
Tissues bioluminescence
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IN VIVO ASSAYS

INFECTION PROGRESSION
Bioluminescence imaging
Bi-Photon microscopy
 
CLINICAL READOUTS
Survival curves
Body weight loss & temperature
Distress
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CLASS II / III PATHOGENS (bacteria, virus, parasites, fungi)

BSL3
Challenge / Infection Assays
PATHOGEN DETECTION & ANALYSIS
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IMMUNO-PHENOTYPING
INNATE & ADAPTATIVE RESPONSES
 
Bacteria, viruses, fungi, parasites
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Immuno_Dendritic cell.png
Dendritic cell
 
 

 

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Naive CD4+ TH cell
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IL-12
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TH 1
STAT4/TBX21

IFNg

Cellular Immunity
IL-4
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TH 2
STAT6/GATA3
IL-4
IL-5
IL-13
Humoral Immunity Allergy
IL-6
IL-21
IL-23
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TH 17
STAT6/RORd /RORg
IL-17
IL-22
IL-21
Inflammation
Autoimmunity
IL-2
TGFb1
ATRA
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TrREG
IL-10
TGFb
Immune Tolerance

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HOST IMMUNE RESPONSE
ADJUVANT POTENCY
VACCINE DEVELOPMENT

 

Our dedicated disease models

Inflammation

Peritonitis (thyoglycollate, LPS, Zymosan, poly-IC)
T cell induced IBD
EAE (multiple sclerosis model)
Genista model - Neutropenia
 

Immuno-Oncology

B16F10, B16F10-Ova melanoma
MC38, CT26 Colorectal cancer
Gliobastoma
E0711 Breast cancer
Tumor models in humanized mice

Infection

Brucella abortus
Bordetella pertussis
Candida albicans
MCMV
Listeria monocytogenes

 

Have a look at our listing of prestations and disease models

BSL3

Purpose

Controlling infectious diseases requires understanding of the relationships between immune cells and pathogens.

Most current studies to understand infectious processes rely on models requiring

  • Infection of cell culture or explants
  • Analysis of pathological phenotypes induced after prior fixation of infected samples obtained from infected mice. The possibility of carrying out those manipulations in a protected confined environment avoids the tissues fixation steps which often impair the possibility of obtaining high-quality anatomical and cellular information. However, very few laboratories are capable of carrying out these experiments.

The Biosafety Level 3 Containment Facility (BSL3) has special engineering and containment features that allow investigators to work safely with pathogens classified as risk group 2 or 3. This facility consists of three laboratory suites corresponding to an animal housing room, a laboratory for in vitro experiments and a bio-imaging area.

Equipments

  • Through PHENOMIX program, we equipped the BSL3 module with a multiphoton confocal microscope that is specially designed to operate in such a confined environment.
  • The Infrastructure program PHENOMIN enabled us to acquire a small animal imaging bioluminescence system IVIS III.
  • Finally, the Infectiopole-Sud program provided us a mouse housing cage system adapted to BSL3 conditions and a cryostat.

Phenotyping tools

All instruments are accessible to trained users based on the scientific and regulatory rules established by scientific and biosecurity and safety manager of BSL3. Although the BSL3 platform was only fully operational in May 2015, it already permitted to set up

  • Vaccine assays,
  • Real-time imaging of immune responses in mice challenged by pathogens,
  • An infection-immunophenotyping analysis pipeline,
  • To test the ability of adjuvants to enhance vaccine efficacy in a mouse model of infection.

Diseases investigation and models

Real-time imaging studies of immune responses in mice challenged by pathogens such as Salmonella or Brucella infection have led to the development of an immunophenotyping analytical pipeline devoted to infection in synergy with our Immunophenotyping module. The analysis of organ distribution of biomolecules engineered to transport and deliver nucleic acids on living animals using an IVIS Lumina III device is also developed in the frame of a contract with a biotech company. Therefore, we can visualize the spread of infection in vivo, isolate and characterize the pathogens, and analyze phenotypic and functional modification of immune system in the course of infections by advanced live-imaging and flow and mass cytometry.

Access and conditions of equipment use

We provide a broad range of instruments and services for academic and industrial customers. An experienced staff of scientists, technical experts and instrument specialist provides instructions for the design, execution and analysis of standardized multiparametric flow, spectral, mass cytometry and advanced microscopy based studies. All these cutting-edge equipment are technically and operationally enrolled in a regular maintenance program with their commercial providers. Moreover, instrument specialists at PHENOMIN are dedicated to the maintenance and tuning of the instruments on a daily basis. The characterization of the cytometer status and performance using CS&T beads for cytometer set up and tracking system, ensure reproducible performance and instrument sensitivity on a day to day basis. All experiments are standardized using applications setting in order to achieve: (1) high consistency of results over time and (2) facilitate data analysis and results validation. All data are archived and stored on dedicated servers. Depending on the technical complexity and availability, the equipment can be handled by our experts or made available for use to trained customers. PHENOMIN has set up an on line instrument tutorial for training external users. PHENOMIN applies two types of price: one for its academic clients on full cost basis and the other one for its industrial customers taking into consideration intellectual property and the transfer of know-how.

Purpose

Real-time imaging studies of immune responses in mice challenged by pathogens (Salmonella infection model) and infection-immunophenotyping analysis pipeline in synergy with immunophenotyping CIPHE facility.

Purpose

Set up vaccine assays and test the ability of adjuvants to enhance vaccine efficacy in a mouse model of infection.

Models

Models and Challenges