Cardiovascular diseases (CVD) are the primary cause of mortality over the world. Major CVD are caused by atherosclerotic plaques, valve dysfunctions, cardiomyopathy, arrhythmia, and heart infections. We provide you with several tests to evaluate functional or anatomical abnormalities related to heart and vessels.
The measurement of various blood metabolites, ions, and enzymes provides a primary screen for function of major metabolic organs such as kidney, liver, gastrointestinal tract, as well as for lipid and glucose homeostasis.
A panel of parameters is proposed, including:
These tests are performed with an AU-480 automated laboratory work station (Beckman Coulter France SAS, Villepinte, France).
Champy MF, Selloum M, Zeitler V, Caradec C, Jung B, Rousseau S, Pouilly L, Sorg T, Auwerx J. Genetic background determines metabolic phenotypes in the mouse. Mamm Genome. 2008 May;19(5):318-31. Epub 2008 Apr 5.
Determination of prothrombine time, activated partial thromboplastin time and fibrinogen activity.
ST4 semi automated coagulometer (Diagnostiqua Stago, Gennevilliers, France).
It is recommended to collect blood by intra-cardiac puncture.
Echocardiography is considered the first-line diagnostic test, from which subsequent imaging or interventions can be recommended.
The Vevo 2100 High-Frequency Ultrasound Systems have been developed specifically for use in small animal imaging and are ideally suited for mouse or rat echocardiography. With center operating frequencies ranging from 15MHz to 50MHz one imaging system can be used to assess cardiac function from embryo to adult in many commonly used small animal models.
Vevo imaging System (VISUALSONICS, Amsterdam, The Netherlands).
Number of animals: 7-9 mice/gender/genotype
Evaluation of the electrical activity of the heart in anesthetized mice.
Experimental reproduction of a cardiac infarction by reversible suturing.
Implantation of a cannula probe to directly measure arterial of ventricular blood pressure in an anesthetized mouse.
Pathological model resulting in average 10% increase in blood pressure but with major fibrosis and left ventricle thickening.
We perform multi-derivation ECG heart in anesthetized mice with 8 probes to generate information about how the electrical activity propagates in 3 dimensions and to determine the electrical axis of the heart. Axis deviations may result from cardiac injury or from a congenital defect.
Electrical heart axis difference and specific electrical transmission issues are determined by multiderivational ECG. Multiderivational ECG profiles of a wild-type mouse (left) and mutant mouse (right). Deviated profiles in the mutant animal suggest a left-heart contraction deficiency as well as rotation of the electrical axis to 90-120⁰ from the normal 30-60⁰.
Any strain background can be used
Electrocardiograph ISO DAM8 amplifier (World Precision Instruments, Sarasota, USA) with ECG AUTO v126.96.36.199 (Emka technologies, USA) and 8 lead positions probes.
We recommend 6 mice per group minimum in same anaesthesia conditions and probes positions
Regular forced exercise can create a situation of cardiac hypertrophy. We utilize a swimming regimen in a warm pool over several weeks to induce cardiac hypertrophy.
Telemetry blood pressure and heart rate: continuous recording of the systolic, diastolic and mean blood pressure and heart rate in conscious mice.
Telemetry EKG: continuous recording of electrocardiogram in conscious mice.
24 channels Data Sciences International acquisition system with 24 RPC-1 telemetric probe
The receiver is the RPC-1 receiver - DSI
-The Data Exchange Matrix - DSI
-The Computer (Dell) - DSI
-The Data Acquisition and Analysis System (Dataquest) - DSI
Telemetry blood pressure and heart rate
The implantable telemetry, PA-C10 Device (transmitter), for blood pressure and heart rate - DSI
The implantable telemetry devices (transmitters) – DSI ETA-F20 Device for Electrocardiogram
Number of animals: 8-10 mice/gender/genotype
Optimal body weight: 30g
Use of hypoxia chambers to induce Pulmonary Arterial Hypertension (PAH) for three of four weeks demonstrates significant resemblance to WHO group III categorized PAH, which includes COPD.
Exposure to chronic hypoxia provides a model of COPD and other PAH disorders in mice. For C57BL/6 mice maintained for 21 days at 0% we see highly significant increases in haematocrit, cardiomyocyte surface area, and right ventricular systolic pressure mimicking human PAH.
Exposure of C57BL/6N mice to 10% O2 for extended periods resulted in (A) weight loss; (B) increased hematocrit; (C) increase right-ventricular systolic pressure (RSVP); and (D) increased cardiomyocyte surface area.
Pathological model resulting in average 37% increase in blood pressure with small decrease of myocardial relaxation.
Pathological model resulting in average 18% increase in blood pressure with thinkening of arteries and left ventricle.
Pathological model resulting in average 17% increase in blood pressure and some left ventricular hypertrophy.
Determination of systolic blood pressure and heart rate in conscious mice by tail-cuff method.
Two apparatus of BP-2000 seriesII 6 channels (Visitech Systems, Apex, North Carolina, USA)
Number of animals: 9 mice/gender/genotype . Maximum age : 8 weeks
Scan arteries and veins with ultrasound to :
The Vevo 2100 High-Frequency Ultrasound System from Vevo Imaging Systems (VISUALSONICS, Amsterdam, The Netherlands).
Number of animals: 7 mice/gender/genotype/experimental group
Identification of specific bacterial loads present in the feces of mice by virtue of quantitative ribosomal 16S DNA sequencing.
For more details, please see our Microbia profiling dedicated section.
See our Gene expression analysis dedicated page to explore our different related tests.
With several months notice, we can procure and provide a variety of different restriction diets for nutritional analyses in conjuction with our other analyses.
We can procure and provide a high sodium diet for vascular, renal, and cardiac challenges.