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It is my great pleasure to invite you to the third CCP Phenogenomics Conference. This year, the conference will be a virtual meeting due to the continuing uncertainty about travel.
The scientific committee has selected two main thematic focuses.
Even though, taking place only in a virtual environment, we believe that the Conference will provide an excellent opportunity to support networking and interactions among the CCP users and experts.
We are looking forward to meeting you virtually in September 2021.
Director of the Czech Centre for Phenogenomics
Institute of Molecular Genetics of the Czech Academy of Sciences"
We invite you to browse through our latest newsletter, whose main topics are
Congenital anomalies (CA) affect 1 in 33 infants worldwide and result in approximately 3.1 million birth defect-related disabilities every year. CA are generally diagnosed only after birth or, more rarely, at late stages of pregnancy. Yet, most of them are determined early during the very first weeks after fertilization. To better understand the pathogenesis of CA at early embryonic stages, researchers from IGBMC (Institut de Génétique et de Biologie Moléculaire et Cellulaire) and PHENOMIN-ICS (PHENOMIN-Institut Clinique de la Souris) proposed to compare three-dimensional (3D) reconstructions of mouse embryos carrying known mutations with their normal littermates at the start of appearance of the different organs. They are first aiming to compare mouse embryos at embryonic day 11.5 (E11.5) which are very similar to human embryos at the 6th week of gestation.
3D imaging is a useful tool to characterize morphological phenotypes, but also to describe the normal anatomy of embryos for educational purposes. Therefore, as a start, the researchers provide to the scientific community the first annotated 3D representation of E11.5 mouse embryo using HREM (high resolution episcopic microscopy). This technology generates images that are comparable to histology in terms of resolution: it allows visualizing the surfaces and volumes of internal organs and cavities, in the nervous system, heart and vasculature, primitive gut, and urogenital system. Each image content is detailed and annotated, inspired by the texts of Karl Theiler (1972) and Matthew Kaufman (1992).
This innovative educational material was developed as part of our participation in the PATHBIO consortium, which aims at designing and developing a series of innovative materials destined for teaching the anatomy, histology, embryology, imaging and pathology of the mouse. We are pleased that it is now freely available online.
And congratulations to the teams!
 Karl Theiler, The House Mouse © Springer 1972
 Matthew H. Kaufman, The Atlas of Mouse Development © Academic Press 1992
This April, an article in Nature Genetics highlights the major contribution of PHENOMIN-ICS in the International Mouse Phenotyping Consortium (IMPC) for the implementation of a comprehensive quality control strategy for alleles in targeted embryonic stem cell clones produced by high throughput. More than 5000 resulting mouse lines were generated with these ES cell clone through a collaborative effort of the different IMPC centers. All of them have the great advantage of having been established in the same C57BL/6N genetic background. Almost 3,700 have a versatile allele that has the advantage of being able to be transformed either into a conditional knockout or into a knockout bearing a LacZ reporter gene. The conditional allele remains a tool of choice for all scientists wishing to perform more detailed analyzes. The total number of genes with a potential conditional allele has been nearly doubled thanks to the effort of the IMPC.
The ultimate goal of IMPC is to generate and phenotype in a standardized manner the KOs of all protein-coding genes to provide key information for the biology and understanding of the development of human diseases. We were able to show that 72% of the KO lines show at least one phenotype, thus revealing a significant pleiotropy. This proportion includes the 39 % showing partial or total lethality. Thanks to this tremendous effort, almost half of the genome is now targeted. Since 2015, the production of KOs from ES cells has been replaced by simple KOs obtained by CRISPR / Cas9 technology. However, in the new era of genome editing, this ES cell-derived collection remains of unique value, as it offers particularly sophisticated and quality-controlled alleles representing a cornerstone of the collective development of a resource of null allele for the complete genome of mammals.
Do you want to take advantage of our expertise to manage the health and safety of your favorite models and to improve data reproducibility ? Take the “fast track” towards protecting the health and safety of your model colonies with the Charles River's upcoming “Quick Insights in Animal Health” event in which PHENOMIN-ICS will share crucial information through the various presentations of track 3 and 6. The free learning series features short, on-demand presentations (each no more than 15 minutes) across six different tracks.
See you online, March 29th !
The French interprofessional research think tank Gircor has just published a Transparency Charter on the use of animals for scientific and regulatory purposes signed by several entities of the scientific community in France, including PHENOMIN.
PHENOMIN is committed to ensuring better transparency by informing on current research and by giving the opportunity to understand the place of the animal models in scientific and biomedical research's progresses, and finally by explaining the conditions under which animal research is practiced.
The French initiative will strengthen the overall vision shared with many European countries and many scientists to support transparency for animal research.
IGBMC, one of Europe's leading biomedical research centers counting 700 employees of 45 nationalities and 47 research teams focusing on the study of human pathologies such as cancer and genetic diseases. The Mouse Clinical Institute (Institut Clinique de la Souris - iCS), the IGBMC's largest technology platform, is ISO 9001 and NFX 50-900 certified. It is the leading center of the national infrastructures PHENOMIN and CELPHEDIA and a key player in the European and global effort to annotate the mouse genome via the INFRAFRONTIER and IMPC consortia.
The IGBMC campus is located on the Illkirch Innovation Park in the suburbs of Strasbourg, France, an exceptional academic and industrial scientific environment that strongly favors collaborations and technology transfer.
For its activities the iCS relies on its IT Development Manager (M/F).
A permanent position to be filled as soon as possible
Maintenance of the intestinal barrier is critical given that it controls the absorption of nutrients, electrolytes, and water from the gut lumen into the blood circulation and further prevents the absorption of toxicluminal substances. Macrophages contribute not only to eliminating pathogens but also to the good functioning of most tissues of the human body (management of dead cells, healing, and maintenance of cardiac functions, arterial and neuronal). PHENOMIN-CIPHE has developed an innovative model of knock-in mice which has been used to induce Macrophages depletion (CD64DTR model). The research teams from the Institut Curie (Paris, France) used it to study role of Macrophages in the integrity and function of the colon epithelium in homeostasis. Authors show that sub-epithelial Macrophages perform a rapid quality check of the fluids absorbed through distal colon epithelial cells, protecting them from being poisoned by luminal fungal metabolites.
COVID-19 research with mouse disease models
We are excited to inform you about INFRAFRONTIER´s new free-of-charge TA Call: COVID-19 Therapeutics Pipeline. Our goal is to provide extensive user support to the selected projects for assessing novel potential vaccine and treatment candidates for COVID-19. The TA service is covered by the INFRAFRONTIER2020 project (Horizon 2020).
INFRAFRONTIER partners (PHENOMIN-CIPHE and CCP) will execute this call by providing viral particles, COVID-19 mouse models, cohort production, and BSL3 pipeline analysis. Further functional analysis and advanced readouts like organ histopathological analysis, multiplex assay profiling of cytokines and chemokines in lung and mouse serum, etc. can be provided on a collaborative basis outside the TA call scope.
For further questions please write to us: firstname.lastname@example.org
PHENOMIN-ICS supports you in acquiring a general knowledge of the major stages of the mouse embryonic development; additionaly O.Wendling and H. Jacobs will train you to be able to design a first-line phenotyping experiment on mouse embryos, to determine a window of lethality in utero and finally to improve your knowledge of imaging techniques adapted to mouse embryos
The training is scheduled online, next March 30 - 31st, and will be conducted in French.
More information at https://imaging-pathbio.sciencesconf.org/
This training aims to provide a general framework to get scientists started using CRISPR-Cas9 for in vivo gene editing in rodents.
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More information at https://cnrsformation.cnrs.fr/in-vivo-crispr-cas9-genome-editing?mc=CRISPR
Lectures - theoretical aspects (3 hrs)
Workshops - practical aspects (3 hrs)
Ces journées ont pour but principal de faciliter les échanges, de promouvoir les contacts et d’aider à la dissémination des connaissances et des compétences sur la génétique de la souris. Elles s’adressent à l’ensemble des personnels s’intéressant aux modèles murins : chercheurs, post-doctorants, étudiants, personnels associés aux plateformes de transgenèse ou d’imagerie et les personnels zootechniques. Pour faciliter ces échanges, les réunions du club des Belles Souris sont informelles et conviviales, en Français et en Anglais, ouvertes à tous et gratuites.
Les orateurs sont d’une part des chercheurs confirmés de renommée internationale et d’autre part de jeunes chercheurs prometteurs. La variété des thématiques abordées dans les présentations favorise les interactions entre scientifiques de domaines de recherche distincts et le développement de nouveaux projets originaux souvent à l’interface entre disciplines différentes.
Le prochain Club des Belles Souris aura lieu le 18 novembre 2021 à Marseille. Si la situation sanitaire le permet, le colloque se tiendra selon un format mixte, en personne sur place dans la salle de conférence du GLM CNRS et en ligne. Le nombre de participants sur place est limité et sera ajusté en fonction de la situation de l’épidémie de Covid-19 en novembre. La liste des participants en présentiel sera établie en respectant l’ordre chronologique d’inscription au colloque.
L’inscription est gratuite mais obligatoire en cliquant ici.
More information at http://stemcells.free.fr/?page_id=1175
Genotyping is often considered as a straightforward and easy step; in reality providing robust, accurate, and fast results is frequently more challenging that it seems.
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More information at https://cnrsformation.cnrs.fr/genotypage-design-a-part-entiere?mc=CRISPR
Attend the French congress in animal science
Les Sciences de l’Animal de Laboratoire tous les ans les scientifiques du domaine autour de thèmes scientifiques, règlementaires, éthiques et techniques. Le 46e Colloque annuel de l'association française se déroulera du 30 Novembre au 02 décembre 2021 dans la ville de Marseille et dont le titre sera “Expérimenter c’est aussi anticiper”. En effet, cette année L'AFSTAL traitera le thème de l’anticipation en vue de la réalisation d’un projet dans son ensemble : éthique, expérimental et scientifique et dediera la première session du colloque à la place de l’animal dans les progrès de la recherche pour répondre à une attente sociétale.
PHENOMIN sera présent à cet incontournable rendez vous français en Sciences de l’Animal de Laboratoire. venez nous rejoindre et nous rencontrer !
More information at https://www.colloque-afstal.com/2020/
This new free edition aims at gathering experts in neurodegenerative diseases with a focus on animal models in Neuroscience Translational Research. Besides, you will be able to interact with all key actors in the field: researchers, clinicians, TTO, biotech, and pharmaceutical companies. This event will also highlight young researcher skills. PHENOMIN will be present to this event.
You could attend in-person as well as online. So, take the opportunity to participate to 3 events in one:
- Hear KOLs from Academia and Industry in plenary and round table sessions and young researchers introducing their work in posters and “my thesis in 180 sec”.
- Initiate R&D collaborative projects during digital B2B meetings
- Increase your network with the virtual poster session
More details on program and registration are coming soon.
SAVE THE DATE !
The complexity of biological systems we study are a key factor impacting research reproducibility. For studies employing transgenic rodent lines, a lack of understanding of genetic backgrounds, as well as genetic drift, genetic contamination, and genotyping inconsistencies, all contribute to the issue of reproducibility in mouse studies. For further insight we are pleased to present the following Charles River-hosted webinar presented by S. Jacquot from PHENOMIN-ICS
PHENOMIN-ICS covers the following topics in this presentation
Genotyping step is crucial in the model creation process as it allows to select animals carrying the good combination of alleles for phenotyping analyses. The different technics are based on the search for a variation in the DNA sequence (in length, composition, quantification...) located at a well-defined place in the genome. PHENOMIN-ICS offers customized genotyping services for knock-out, knock-in, transgenic or any other genetically modified murine model. Different sample types can be processed, including tail biopsies, ear punches, embryonic yolk sac, as well as non-invasive biopsies. We provide a detailed genotyping protocol to all our users on request.
More information at http://www.phenomin.fr/en-us/contact/
CRISPR-Cas9 is a rapid and cost effective technology to generate mouse and rat mutant models, however, its impact on the genome still requires careful consideration. For further insight we are pleased to present the following Charles River-hosted and Labroots-hosted webinars presented by G. Pavlovic and M-C Birling from PHENOMIN-ICS
PHENOMIN-ICS works with you from concept to model creation using homologus recombination, latest CRISPR/Cas9 and RNAi technology, and either other trangenic technics to deliver the most relevant model for your application. The effective research with rodent models depends on finding the best appropriated model for your project. How can we help you? Request a quote or email us.
More information at http://www.phenomin.fr/en-us/contact/
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