PHENOMIN offers 37 different services, distributed by the three partners (ICS, TAAM and CIPHE) and operated in a coordinated manner. These services peformed by our experts benefit users in genetic engineering, model validation experiments, state-of-the-art zootechnic and phenotypic approaches, archiving and distribution.
Charles River Europe and Phenomin-ICS Launch New Collaboration for Model Creation
This exciting partnership offers our European clients a complete integrated solution for mouse and rat models, from creation to validation. Our combined in vitro and in vivo expertise shapes a broad portfolio of design (ES cell, CRISPR/Cas9, random DNA insertion), breeding and associated services to create and maintain the most relevant models for your studies.Rely our industry-leading germ line transmission, strict quality controls, fast turnaround and dedicated project managers to help you successfully reach your goals.
Genetic Engineering Design Options
CRISPR-Cas9 is a rapid and cost effective technology to generate mouse and rat mutant models. Now we can offer the generation of knock-out models though direct injection into fertilized mouse oocytes of CRISPR sgRNA and Cas9 mRNA. This approach allows to obtain models in shorter timelines and with reduced costs compared to gene targeting by homologous recombination in ES cells.
Our offer involves:
- Design of an optimized strategy including prediction of off-target sites
- Preparation of sgRNA and Cas9 mRNA
- Injection of sgRNA and Cas9 mRNA into zygotes, to generate F0 mutated animals
- Genotyping of F0 animals and delivery of F0 candidate
- Optional steps:
- Off-target analysis in F0 founder mice (optional)
- Germline breeding of F0 founder mouse to achieve germ line transmission (optional)
- Off-target analysis in germline transmitted F1 mice (optional)
The rat model offers better clinical characterization approaches than mouse model in some cases.
Because rat shares many physiological similarities with humans, the rat has long been a model favored by physiologists, pharmacologists and neuroscientists.
Their larger size allows to perform several experiments, surgical procedures and to monitor physiological parameters that are difficult or impossible in mice. Rat is a key model for cardiovascular disease studies: atherosclerosis, hypertension, stroke models, not only because of its size, but also because of its physiology. By their ability to learn and accomplish sophisticated behavioral tasks, rats are relevant model for nervous system diseases.
Now, CRISPR/Cas9 genome editing opens a new area in rat genetics.
- 1- Jacob, H.J. & Kwitek, A.E. (2002) Multifactorial genetics: Rat genics: attaching physiology and pharmacology to the genome, Nat. Rev. Gen., 3(1), 33-42.
- 2-Cozzi J. et al., (2008) Use of genetically modified rat models for translational medicine, Drug Discovery Today, 13(11/12), 488-94.
- 3- Aitman TJ et al., (2008) Nat Genet. Progress and prospects in rat genetics: a community view. 2008 May; 40(5):516-22.
CRISPR/Cas 9 frequently asked questions
CELPHEDIA WP1 workgroup gathers experts using CRISPR/Cas9 system in various animal models including mouse, rat, zebrafish and drosophila. By sharing our knowledge, experience and an extensive bibliographic review, we have worked together to write some recommendations for CRISPR/cas9 system. Our goal is to help scientists who want to use this technology for gene editing by summarizing current advances on many technical aspects, from the RNA guide optimized design to the genotyping analysis and the validation of the newly generated models.
Archiving and Distribution
- Cryopreservation (Embryos-sperm)
- Distribution (Embryos-sperm, living mice)
Genetic Engineering and Model Validation
- Trangenesis (plasmid, BAC)
- Targeted Genetic Engineering and Model Validation (ES cells)
- Genetic Engineering and Model Validation through IKMC recombinated ES cells
- Production of recombinated ES cells
- Clinical phenotyping
- In vivo imaging
- Drug screening, derisking strategy
- Bio imaging under BSL3 conditions
- In vivo disease models
- Health sanitary controls
- Maintenance of heath status
- Housing and breeding
- Validation of mouse models