PHENOMIN, as a large scale national infrastructure for Biotechnology and Health, provides services for all French laboratories.

PHENOMIN offers 37 different services in genetic engineering, model validation experiments, state-of-the-art zootechnic and phenotypic approaches, archiving and distribution. These services benefit to users who conduct genetic researches combining generation, validation and phenotyping analysis of mutant mice model (physiological, behavioral and anatomical analysis).

Oncology services 

PHENOMIN has developed expertise for oncology studies using mouse models for cancer progression and/or initiation.  We are available to discuss projects, in close collaboration with users in order to customize any study to the scientific objectives applying the best adhesion to 3R rules. Our services:

  • Progression and initiation models 
  • Bioluminescent tumor cells
  • Phenotyping and immunophenotyping

Have a look to our whole offer !

 

CRISPR-Cas9 rodent genome editing

PHENOMIN offers the generation of knock-out models though direct injection into fertilized oocytes of CRISPR sgRNA and Cas9 mRNA. This approach allows to obtain models in shorter timelines and with reduced costs compared to gene targeting by homologous recombination in ES cells.

Modeling human disease in rodents by CRISPR/Cas9 genome editing. Birling MC, Herault Y, Pavlovic G. Mamm Genome. 2017 Jul 4.View on Pubmed

Our offer involves:

  • Design of an optimized strategy including prediction of off-target sites
  • Preparation of sgRNA and Cas9 mRNA
  • Injection of sgRNA and Cas9 mRNA into zygotes, to generate F0 mutated animals
  • Genotyping of F0 animals and delivery of F0 candidate
  • Optional steps (for mouse models):

-Off-target analysis in F0 founder mice (optional)

-Germline breeding of F0 founder mouse to achieve germ line transmission (optional)

-Off-target analysis in germline transmitted F1 mice (optional)

Let us give you the advantages and drawbacks regarding the suse of CRISPR/Cas9 in generating mouse and rat models. 

 

 

 

Archiving and Distribution

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  • Cryopreservation (Embryos-sperm)
  • Distribution (Embryos-sperm, living mice)
  • Rederivation

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Genetic Engineering and Model Validation

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  • Trangenesis (plasmid, BAC)
  • Targeted Genetic Engineering and Model Validation (ES cells)
  • Genetic Engineering and Model Validation through IKMC recombinated ES cells
  • Production of recombinated ES cells

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Phenotyping

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  • Clinical phenotyping
  • In vivo imaging
  • Drug screening, derisking strategy
  • Bio imaging under BSL3 conditions
  • In vivo disease models

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Zootechny

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  • Health sanitary controls
  • Maintenance of heath status
  • Housing and breeding
  • Validation of mouse models
  • Revitalization

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