PHENOMIN offers 37 different services, distributed by the three partners (ICS, TAAM and CIPHE) and operated in a coordinated manner. These services peformed by our experts benefit users in genetic engineering, model validation experiments, state-of-the-art zootechnic and phenotypic approaches, archiving and distribution.
CRISPR-Cas9 is a rapid and cost effective technology to generate mouse and rat mutant models. Now we can offer the generation of knock-out models though direct injection into fertilized mouse oocytes of CRISPR sgRNA and Cas9 mRNA. This approach allows to obtain models in shorter timelines and with reduced costs compared to gene targeting by homologous recombination in ES cells.
Our offer involves:
Because rat shares many physiological similarities with humans, the rat has long been a model favored by physiologists, pharmacologists and neuroscientists.
Their larger size allows to perform several experiments, surgical procedures and to monitor physiological parameters that are difficult or impossible in mice. Rat is a key model for cardiovascular disease studies: atherosclerosis, hypertension, stroke models, not only because of its size, but also because of its physiology. By their ability to learn and accomplish sophisticated behavioral tasks, rats are relevant model for nervous system diseases.
Now, CRISPR/Cas9 genome editing opens a new area in rat genetics.
CELPHEDIA WP1 workgroup gathers experts using CRISPR/Cas9 system in various animal models including mouse, rat, zebrafish and drosophila. By sharing our knowledge, experience and an extensive bibliographic review, we have worked together to write some recommendations for CRISPR/cas9 system. Our goal is to help scientists who want to use this technology for gene editing by summarizing current advances on many technical aspects, from the RNA guide optimized design to the genotyping analysis and the validation of the newly generated models.