TRAINING COURSE Mycoplasmes detection in cell culture
March 29, 2017
Engineers and technicians currently cultivating cell lines or planning to cultivate cell lines.
None, except understand French (course will be given in French)
Interactive courses (5h)
Workshop 2 h
Starting from concrete cases, trainees will be asked to analyze the situation in order to suggest an action plan
Registration and more informations: Download the program and pre regristration form
SPECIAL EVENT Webinar CRISPR/Cas9 in Rodent Animals
March 30, 2017
CRISPR/Cas9: The Good, the Bad and the Ugly
March 30, 2017
3:00 PM CEST/9:00 AM EST
In the past years, CRISPR/Cas9 has shown to be a real revolution for generation of genetically engineered animals and a real hope for genetic therapy in humans. This powerful genome editing technology is working in a very large panel of species from plants to mammals. In rodents, numerous knock-out or point mutations models were easily generated with this approach. Among other advantages, it is fast (to obtain founder animals), quite easy (in comparison to ES route) and cost-effective. It also allows accessing a large panel of background and thus promise to be a powerful tool for polygenic studies.
In this session, we will present our results for generating complex mouse or rat models. We developed the CRISPR Mediated REarrangement (CRISMERE) strategy, which takes advantage of the CRISPR/Cas9 system, to generate easily most of the desired structural variant & CNV rearrangements. We were indeed able to achieve deletions, duplications, and inversions of genomic regions as large as 24.4 Mb in rat and mouse (the good).
We also used CRISPR/Cas9 to develop knock-in or conditional lines. We will show the actual limitations of this system for the modification of “big” genomic sequences in mouse and rat (the bad) and discuss possible solutions and explanations.
Finally, we will also show that very careful validation of the lines generated by CRISPR/Cas9 is requested (the ugly) and how some unexpected event can be used to generate targeted overexpressing models.
More information at http://www2.criver.com/l/60962/2017-03-08/ch9pxs
TRAINING COURSE 2nd Programmable Nucleases Course
April 3 - 7, 2017
Vestec, Czech Republic
This course, jointly provided by the Czech Centre for Phenogenomics and PHENOMIN/ICS Strasbourg, both members of INFRAFRONTIER, is designed to give practical, hands-on training in current technologies and methods employing CRISPR/Cas9-mediated genome manipulations in the generation of mice mutants. Participants will gain theoretical and practical knowledge from our expert instructors, have ample opportunity for discussion and can bring their own targeting projects to work on during the bioinformatics session. The course has been designed for participants with an intermediate knowledge of molecular biology.
More information at https://www.phenogenomics.cz/2016/12/transgenesis-course-in-april/
SPECIAL EVENT 2d European Advanced School for Mouse Phenogenomics
June 12 - 16, 2017
Would you like to learn how to choose, create and analyse your best mouse model?
Do you have a sufficient background in mouse functional genomics to lead as best your research?
Then, come to the heart of Europe to be involved - together with other students and researchers from all over the world - in opened discussion, in constructive analysis of your own research and in building conceptual scientific advances.This unique international event will provide the attendees with an innovative program focused on the best practices in the use of the mouse model for biomedical and basic research
24 experts from European landscape will be present to:
You will gain crucial skills required to use as best mutant mice (best experimental design, scientific and ethic consideration for you best experimental design, awareness for improving robustness of your scientific data,..)
The target audience consists of engineers and/or researchers who directly or indirectly use mouse as animal model for scientific research.
We encourage both industrial and academic researchers, postdocs and PhDs from all Europe to attend this school.
CNRS researchers: FREE of charge
PhD applicants: 360€ (FREE of charge for the students from the University of Strasbourg and University of Aix-Marseille)
Applicants from governmental institutions: 600€
Applicants from private institutions or companies: 1680€
Deadline for application : April 28th 2017 (Early bird dead line : March 31st)
More information on program and registration: https://advanced-school.phenomin.eu/
Contact : firstname.lastname@example.org
More information at https://advanced-school.phenomin.eu/
TRAINING COURSE Small animal imaging: evolution, potential, limits
November 6 - 10, 2017
The course is conducted in French
CNRS formation entreprises
TRAINING COURSE In vivo CRISPR-Cas9 genome editing
November 9 - 10, 2017
PHENOMIN's training aims to provide a general framework to get scientists started using CRISPR/Cas9 for in vivo gene editing in rodents.
The main topics are:
CNRS formation entreprise