Upcoming training courses
TRAINING COURSE In vivo CRISPR-Cas9 genome editing
March 14 - 15, 2018
PHENOMIN's training aims to provide a general framework to get scientists started using CRISPR/Cas9 for in vivo gene editing in rodents.
The main topics are:
- Learn more about gene editing and how it works
- Hear about current advances on many technical aspects
- Optimize the RNA guide design to the genotyping analysis (bioinformatics workshop).
- Highlight crucial issue in your own scientific project.
The course in conducted in English.
One other session shceduled in November 2018.
Acces to Detailed program and registration procedure
CNRS formation entreprise
More information at https://cnrsformation.cnrs.fr/stage-18188-In-vivo-CRISPR-Cas9-genome-editing.html?stage=18188&mc=mouse
TRAINING COURSE Primary phenotyping of mouse embryos
March 27 - 29, 2018
This training is aimed at providing a theoretical and practical background knowledge destined for researchers and engineers that are willing to acquire primary expertise in mouse development. The topics will cover dissection of post implant embryos, histology, whole mount Lac Z staining and confocal imaging of whole embryos.
Researchers, engineers, scientists. Need to have the basic knowledge in animal biology to understand mouse development physiology and the biological technics for phenotyping.
- Phenotyping of mouse embryos
- Determining the window of lethality of mutant mice in utero
- Introduction to Mouse embryonic development
- Classical histology (Fixation, embedding, orientation, sectioning and staining),
- Immunohistochemistry and in situ hybridization
- Imaging technics (HREM, micro CT, OPT)
- High frequency echography to visualize and quantify embryonic development
- The in-vitro culture of mouse embryos/ drug testing
- Confocal macroscopy: application in embryology
- Collection and fixation of mouse embryos and placentas at E10.5 (mid gestation stage)
- Whole mount Lac Z staining of E10.5 embryos (Step1)
- Observation of embryos by echography
- Observation of embryos by micro CT, OPT (potentialy)
- Whole mount Lac Z staining of E10.5 embryos (step 2) + observation
- Collection of mouse embryos at E8.5 (early embryonic stage)
- Collection of mouse fetuses and placentas at E18.5 (just before birth)
- Observation of embryos at the confocal macroscope
- Visualization of histological sections at different stages of development. Example of abnormalities.
More information at https://sfc.unistra.fr/formations/chimie-bio_-_experimentation-animale-primatologie_-_primary-phenotyping-of-mouse-embryos_-_1162/
TRAINING COURSE Small animal imaging: evolution, potential, limits
November 12 - 16, 2018
- Understand potential and limits of different imaging modalities for small animal: biophotonic imaging (bioluminescence, fluorescence 2D and 3D), X rays (X scanner), echography coupled to photoacoustic imaging
- Be able to select the most suitable approach to address a biological question (phenotyping, functional exploration, biopharmaceutical research...)
The course is conducted in French
Acces to detailed program and registration procedure
CNRS formation entreprises
More information at http://cnrsformation.cnrs.fr/stage-18206-Imagerie-du-petit-animal--evolution%2C-potentiel%2C-limites.html?mc=imagerie