Upcoming training courses

TRAINING COURSE Mycoplasmes detection in cell culture
March 29, 2017
Illkirch, France

Objectives

  • Realize the importance of tracability and quality control in cell culture
  • Be aware of the problem of mycoplasma contamination
  • Realize the diversity of mycoplasma detection methods and be able to choose techniques adapted to each case
  • Know how to prevent mycoplasma contaminations

Audience

Engineers and technicians currently cultivating cell lines or planning to cultivate cell lines.

Prerequisites

None, except understand French (course will be given in French)

Program

Interactive courses (5h)

  • Introduction on cell line culture
  • How to establish cell lines and key steps in the culture
  • Importance of tracability and quality control
  • Mycoplasma: definitions and classifications- origin of contamination- effects of contamination- detection methods- treatment and limits- preventions and actions needed

Workshop 2 h

Starting from concrete cases, trainees will be asked to analyze the situation in order to suggest an action plan


Registration and more informations: Download the program and pre regristration form

PHENOMIN's contact: wattenho@igbmc.fr

More information at https://cnrsformation.cnrs.fr/stage-17280-Detection-des-mycoplasmes-dans-les-cultures-de-lignees-cellulaires.html?mc=mycoplasmes


SPECIAL EVENT Webinar CRISPR/Cas9 in Rodent Animals
March 30, 2017
On line

CRISPR/Cas9: The Good, the Bad and the Ugly
March 30, 2017
3:00 PM CEST/9:00 AM EST

In the past years, CRISPR/Cas9 has shown to be a real revolution for generation of genetically engineered animals and a real hope for genetic therapy in humans. This powerful genome editing technology is working in a very large panel of species from plants to mammals. In rodents, numerous knock-out or point mutations models were easily generated with this approach. Among other advantages, it is fast (to obtain founder animals), quite easy (in comparison to ES route) and cost-effective. It also allows accessing a large panel of background and thus promise to be a powerful tool for polygenic studies.

In this session, we will present our results for generating complex mouse or rat models. We developed the CRISPR Mediated REarrangement (CRISMERE) strategy, which takes advantage of the CRISPR/Cas9 system, to generate easily most of the desired structural variant & CNV rearrangements. We were indeed able to achieve deletions, duplications, and inversions of genomic regions as large as 24.4 Mb in rat and mouse (the good).

We also used CRISPR/Cas9 to develop knock-in or conditional lines. We will show the actual limitations of this system for the modification of “big” genomic sequences in mouse and rat (the bad) and discuss possible solutions and explanations.

Finally, we will also show that very careful validation of the lines generated by CRISPR/Cas9 is requested (the ugly) and how some unexpected event can be used to generate targeted overexpressing models.

Presenters

  • Guillaume Pavlovic, PhD, Head of the Genetic Engineering and Model Validation Department, PHENOMIN-ICS
  • Jean Cozzi, PhD, Embryology Laboratory Manager, Charles River

To register

http://www2.criver.com/l/60962/2017-03-08/ch9pxs

 

More information at http://www2.criver.com/l/60962/2017-03-08/ch9pxs


TRAINING COURSE 2nd Programmable Nucleases Course
April 3 - 7, 2017
Vestec, Czech Republic

This course, jointly provided by the Czech Centre for Phenogenomics and PHENOMIN/ICS Strasbourg, both members of INFRAFRONTIER, is designed to give practical, hands-on training in current technologies and methods employing CRISPR/Cas9-mediated genome manipulations in the generation of mice mutants. Participants will gain theoretical and practical knowledge from our expert instructors, have ample opportunity for discussion and can bring their own targeting projects to work on during the bioinformatics session. The course has been designed for participants with an intermediate knowledge of molecular biology.

More information at https://www.phenogenomics.cz/2016/12/transgenesis-course-in-april/


SPECIAL EVENT 2d European Advanced School for Mouse Phenogenomics
June 12 - 16, 2017
Goersdorf, France

Would you like to learn how to choose, create and analyse your best mouse model?

Do you have a sufficient background in mouse functional genomics to lead as best your research?

Then, come to the heart of Europe to be involved - together with other students and researchers from all over the world - in opened discussion, in constructive analysis of your own research and in building conceptual scientific advances.This unique international event will provide the attendees with an innovative program focused on the best practices in the use of the mouse model for biomedical and basic research

 

24 experts from European landscape will be present to:

  • Exchange and discuss your major on-going works, scientific and methodological issues.
  • Share knowledge and expertise, transfer new concepts and innovative methods

You will gain crucial skills required to use as best mutant mice (best experimental design, scientific and ethic consideration for you best experimental design, awareness for improving robustness of your scientific data,..)

The target audience consists of engineers and/or researchers who directly or indirectly use mouse as animal model for scientific research.

We encourage both industrial and academic researchers, postdocs and PhDs from all Europe to attend this school.

 

CNRS researchers: FREE of charge

PhD applicants: 360€ (FREE of charge for the students from the University of Strasbourg and University of Aix-Marseille)

Applicants from governmental institutions: 600€

Applicants from private institutions or companies: 1680€

 

Deadline for application : April 28th 2017 (Early bird dead line : March 31st)

More information on program and registrationhttps://advanced-school.phenomin.eu/ 

Contact advanced-school@phenomin.eu 

More information at https://advanced-school.phenomin.eu/


TRAINING COURSE Small animal imaging: evolution, potential, limits
November 6 - 10, 2017
Orleans, France

Objectives:

  • Understand potential and limits of different imaging modalities for small animal: biophotonic imaging (bioluminescence, fluorescence 2D and 3D), X rays (X scanner), echography coupled to photoaccoustic
  • Be able to select the most suitable approach to address a biological question (phenotyping, functional exploration, biopharmaceutical research...)

The course is conducted in French

Acces to detailed program and registration procedure

CNRS formation entreprises

PHENOMIN's contact:
stephanie.lerondel@cnrs-orleans.fr 

More information at http://cnrsformation.cnrs.fr/stage-17142-Imagerie-du-petit-animal--evolution%2C-potentiel%2C-limites.html?mc=imagerie


TRAINING COURSE In vivo CRISPR-Cas9 genome editing
November 9 - 10, 2017
Illkirch, France

Objectives

PHENOMIN's training aims to provide a general framework to get scientists started using CRISPR/Cas9 for in vivo gene editing in rodents.

The main topics are:

  • Learn more about gene editing and how it works
  • Hear about current advances on many technical aspects
  • Optimize the RNA guide design to the genotyping analysis (bioinformatics workshop).
  • Highlight crucial issue in your own scientific project.

Acces to Detailed program and registration procedure

CNRS formation entreprise

PHENOMIN's contact:
bedu@igbmc.fr

More information at http://cnrsformation.cnrs.fr/stage-17276-In-vivo-CRISPR-Cas9-genome-editing.html?mc=CRISPR-Cas9