Our mission is to develop the most adapted, highly physiologically relevant animal models for your research program. The genetic engineering services are flexible and extensive to help you generate your custom-made mouse model, from the design of vectors construction to the functional validation of the mouse model. Additional services are proposed for genotyping. Our deliverables are transgenic or chimera mice, in SPF or SOPF status.
R&D program: new engineering tools, lentigenesis
All mouse strains and lines provided by PHENOMIN are available on this page.
R&D program: ES cell differentiation, rat ES cells
The gene expression service validates the consequences of the genetic modifications introduced in vivo. Gene expression analyses are specifically pertinent to select transgenic lines established by pronuclear integration (transgene localization, expression level of the transgene, copy number determination, ...), to validate genetically modified mouse models (expected expression of conditional alleles, level of transcripts, splice variants, hypomorphic effects, cell/tissue specific gene inactivation, ...) and to correlate the gene expression analysis with the phenotype of the animals upon clinical evaluation.
|Gene expression validation and copy number determination||Functional validation|
|Northern Blot, Southern Blot||Immunohistochemistry|
|ddPCR (as replacement of qPCR)||Western Blot|